인문학
사회과학
자연과학
공학
의약학
농수해양학
예술체육학
복합학
DBpia AI
AI 에이전트
AI 아이디어
AI 리더
AI 뷰어
Citeasy
크롬 서지관리 다운로드
지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
초록·키워드
Abstract The escalating crisis of lethal multidrug resistance (MDR) pathogens and the alarming rise of resistance to last-resort antibiotics necessitate urgent exploration of innovative green therapeutic solutions. This study investigates the biogenic synthesis of silver nanoparticles (AgNPs) utilizing aqueous leaf extract from Moringa oleifera (MO) and evaluates their antibacterial efficacy against Gram-negative nosocomial pathogens. Among 150 nosocomial isolates, 3% demonstrated susceptibility to all tested antibiotics, underscoring the severity of the resistance dilemma. The antibacterial potential of the synthesized MO-AgNPs was assessed alongside their cytotoxic effects on a normal mouse liver cell line (BNL). The biosynthesis process involved the reduction of silver nitrate to silver using the MO leaf extract. Comprehensive characterization techniques—including UV-Vis spectroscopy, Fourier Transform Infrared (FTIR) spectroscopy, Zeta potential analysis, Dynamic Light Scattering (DLS), X-ray Diffraction (XRD), Scanning Electron Microscopy with Energy Dispersive X-ray (SEM-EDX), and Transmission Electron Microscopy (TEM) confirmed the formation of spherical MO-AgNPs with an average crystalline size of 12.89 nm. Antibacterial activity was evaluated via the broth microdilution method, revealing that MO-AgNPs exhibited bactericidal effects at a minimum inhibitory concentration (MIC) of ≤ 8 µg/mL for 80% of the pathogens, including 62% of difficult-to-treat (DTR) pathogens and over 90% among antibiotic class resistant groups, including colistin (Col R), extended-spectrum cephalosporins (ECR), fluoroquinolones (FQR), and carbapenems (CR). Importantly, these MICs remained below the cytotoxic threshold (IC 50 = 13.47 µg/mL). These findings highlight the potential of biosynthesized MO-AgNPs as a novel broad-spectrum antibacterial agent, presenting a promising green therapeutic strategy to combat resistant bacterial infections.
인공지능 문자 인식 모델을 통해 추출된 텍스트로, 일부 오타나 오류가 포함될 수 있으나 지속적으로 개선 중입니다.
오류를 발견하셨다면 해당 부분을 드래그한 후 ' 를 통해 신고해주세요.
오류를 발견하셨다면 해당 부분을 드래그한 후 ' 를 통해 신고해주세요.
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