인문학
사회과학
자연과학
공학
의약학
농수해양학
예술체육학
복합학
DBpia AI
AI 에이전트
AI 아이디어
AI 리더
AI 뷰어
Citeasy
크롬 서지관리 다운로드
지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
초록·키워드
Infections caused by drug-resistant bacteria, particularly Gram-negative species, represent one of the most significant global public health challenges. The outer membrane (OM) is a crucial target for the development of drugs against Gram-negative bacteria. For the confirmation of the mechanism of OM-targeted drugs, the evaluation of OM damage is necessary. In this study, we optimized the method for detecting OM damage employing N-phenyl-1-naphthylamine (NPN) and ethidium bromide (EtBr) as probes in Escherichia coli (E. coli), including the bacterial loading, probe concentration, and incubation time. In addition, three OM-targeted compounds with distinctive mechanisms: polymyxin B, ACHN-975, and IMB-0042, were used to investigate the advantages and problems of two fluorescent probes. The compound fluorescence and quenching effect on the probes were detected. It was found that IMB-0042 caused fluorescence quenching on NPN. The treatment time of compounds with different OM damage mechanisms had a significant impact on the detection. For polymyxin B-treated E. coli cells, which directly disrupts OM, significant fluorescence changes were observed with both probes at short (30 min) and long durations (1-5 h). In contrast, compounds ACHN-975 and IMB-0042, which inhibit OM biosynthesis, showed detectable fluorescence only at long durations. In summary, this study presents a detailed scheme for the detection of OM damage induced by different antibiotics based on NPN and EtBr.
인공지능 문자 인식 모델을 통해 추출된 텍스트로, 일부 오타나 오류가 포함될 수 있으나 지속적으로 개선 중입니다.
오류를 발견하셨다면 해당 부분을 드래그한 후 ' 를 통해 신고해주세요.
오류를 발견하셨다면 해당 부분을 드래그한 후 ' 를 통해 신고해주세요.
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