인문학
사회과학
자연과학
공학
의약학
농수해양학
예술체육학
복합학
DBpia AI
AI 에이전트
AI 아이디어
AI 리더
AI 뷰어
Citeasy
크롬 서지관리 다운로드
지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
초록·키워드
The widespread occurrence of macrolide antibiotics in aquatic environments poses serious ecological and public health concerns, highlighting the need for reliable, accessible, and validated analytical methods. In this study, a rapid and cost-effective high-performance liquid chromatography method with photodiode array detection (HPLC-PDA) was developed and validated for the simultaneous determination of azithromycin (AZM), clarithromycin (CLR), and erythromycin (ERY) in wastewater. Chromatographic separation was achieved on an Agilent Zorbax Eclipse C18 column (250 × 4.6 mm, 5 µm) at 35 °C using a mobile phase of acetonitrile and 10 mM NaH<sub>2</sub>PO<sub>4</sub> buffer (60 : 40, v/v, pH 6.0), flow rate 0.8 mL min<sup>-1</sup>, and injection volume 10 µL. Retention times were 3.76, 4.91, and 6.23 min for AZM, CLR, and ERY, respectively, with baseline resolution (<i>R</i> <sub>s</sub> > 1.5). The method exhibited excellent linearity over 2-15 µg mL<sup>-1</sup> for all analytes (<i>R</i> <sup>2</sup> = 0.997-0.999). Limits of detection (LOD) were 5-6 µg L<sup>-1</sup> and limits of quantification (LOQ) were 17-20 µg L<sup>-1</sup>, meeting AOAC (Association of Official Analytical Chemists) performance standards. Intra- and inter-day precision (%RSD) were below 2.0%, and mean recoveries from spiked wastewater samples ranged from 99.1% to 104.5%. Minor deliberate changes in pH (±0.2) or flow rate (±0.1 mL min<sup>-1</sup>) produced negligible effects (<2% deviation), confirming the robustness of the method. Matrix effects were minimal (<3%), demonstrating that the wastewater matrix did not interfere with analyte detection. Application of the validated method to hospital and livestock wastewater samples revealed macrolide concentrations up to 13.36 mg L<sup>-1</sup> (CLR), 11.27 mg L<sup>-1</sup> (ERY), and 7.80 mg L<sup>-1</sup> (AZM). The developed HPLC-PDA method is therefore suitable for routine environmental monitoring, providing a simple and reliable tool for assessing macrolide antibiotic contamination in wastewater.
인공지능 문자 인식 모델을 통해 추출된 텍스트로, 일부 오타나 오류가 포함될 수 있으나 지속적으로 개선 중입니다.
오류를 발견하셨다면 해당 부분을 드래그한 후 ' 를 통해 신고해주세요.
오류를 발견하셨다면 해당 부분을 드래그한 후 ' 를 통해 신고해주세요.
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