인문학
사회과학
자연과학
공학
의약학
농수해양학
예술체육학
복합학
DBpia AI
AI 에이전트
AI 아이디어
AI 리더
AI 뷰어
Citeasy
크롬 서지관리 다운로드
지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
초록·키워드
Impaired intestinal barrier function is a precursor to various metabolic diseases which can occur when the intestinal epithelium is directly exposed to certain dietary components. Previous studies have demonstrated the barrier disruptive effect of artificial sweeteners such as sucralose and saccharin, in the intestinal epithelium. In the present study, we aimed to evaluate the impact of 3 non-synthetic sweeteners, stevia, miraculin, and mogroside V, on Caco-2 monolayer barrier integrity, reactive oxygen species (ROS) production, and tight junction protein expression as compared to the artificial sweetener saccharin. Miraculin and mogroside V exerted a significant impact on cell numbers with decreased cell viability at both 24 and 48 h. ROS production was also significantly increased by miraculin and mogroside V and epithelial barrier function, assessed by transepithelial electrical resistance and FITC-dextran leak, was significantly disrupted with both miraculin and mogroside V. Stevia exhibited no effect on epithelial cell viability, ROS accumulation or barrier function, despite a range of concentrations investigated. Knockdown of the sweet taste receptor, T1R3, significantly attenuated miraculin- and mogroside V-induced loss of cell viability, ROS accumulation and barrier disruption suggesting a T1R3-dependent mechanism. Gene expression analysis revealed differential regulation of cell junction-related genes by mogroside V and miraculin with upregulated expression of genes such as CAV1, CLDN2 and CLDN10, and downregulated expression of genes such as CAV3 and CDH2. These findings demonstrate that miraculin and mogroside V, but not stevia, negatively regulate ROS formation and intestinal epithelial barrier function in a T1R3-dependent manner and potentially through modulation of tight junction proteins. This highlights the need for further investigation into the long-term dietary implications of natural sweeteners, particularly miraculin and mogroside V, on gut health.
인공지능 문자 인식 모델을 통해 추출된 텍스트로, 일부 오타나 오류가 포함될 수 있으나 지속적으로 개선 중입니다.
오류를 발견하셨다면 해당 부분을 드래그한 후 ' 를 통해 신고해주세요.
오류를 발견하셨다면 해당 부분을 드래그한 후 ' 를 통해 신고해주세요.
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