인문학
사회과학
자연과학
공학
의약학
농수해양학
예술체육학
복합학
DBpia AI
AI 에이전트
AI 아이디어
AI 리더
AI 뷰어
Citeasy
크롬 서지관리 다운로드
지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
초록·키워드
Monitoring antibiotic resistance levels is essential to preventing its spread in pig farms. This is usually done through tests to assess phenotypic and genotypic resistance, although air analysis in farms could also be useful for obtaining this information. This study examined genotypic and phenotypic resistance levels in pathogenic E. coli from pig farms from Northwest of Iberian Peninsula and investigated the potential of air sampling as a tool for estimating antibiotic resistance. Rectal swabs were collected from weaned piglets, along with air samples from their housing areas. Bacteria with potential to cause diarrhea were identified from the swabs, followed by phenotypic antibiotic susceptibility testing and screening for resistance genes targeting eight antibiotic families. The presence of these genes was also analyzed in air samples using qPCR. All isolated pathogenic E. coli exhibited phenotypic resistance to at least two families of antibiotics, with aminoglycosides and lipopeptides being the families to which the highest percentage of resistant E. coli was detected (p < 0.05). In terms of genotypic resistance, genes encoding resistance to aminoglycosides, penicillins, and cephalosporins were the most frequently detected (p < 0.05), whereas those associated with lipopeptides, quinolones, and carbapenems were the least common (p < 0.05). Both phenotypic and genotypic resistance tended to cluster according to the origin farm, although positive associations between resistance genes were observed, with tet(A) and blaCTX−universal being the most frequently associated with others. Regarding air samples, there was poor concordance between the detection of resistance genes in air and the levels of genotypic and phenotypic resistance on the farm (k < 0.4), although this seems to be influenced by the different detection patterns of the evaluated genes in air. Pathogenic E. coli from pig farms exhibited a significant level of phenotypic and genotypic resistance to antibiotics, with resistance to aminoglycosides being particularly notable. Additionally, there was a prominent co-occurrence of genes encoding resistance to multiple antibiotic families. The effectiveness of air sampling for estimating farm-level antibiotic resistance seems to be influenced by differences in gene detection performance in this type of sample.
인공지능 문자 인식 모델을 통해 추출된 텍스트로, 일부 오타나 오류가 포함될 수 있으나 지속적으로 개선 중입니다.
오류를 발견하셨다면 해당 부분을 드래그한 후 ' 를 통해 신고해주세요.
오류를 발견하셨다면 해당 부분을 드래그한 후 ' 를 통해 신고해주세요.
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