인문학
사회과학
자연과학
공학
의약학
농수해양학
예술체육학
복합학
DBpia AI
AI 에이전트
AI 아이디어
AI 리더
AI 뷰어
Citeasy
크롬 서지관리 다운로드
지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
초록·키워드
Boerhavia procumbens Banks ex Roxb. (Nyctaginaceae) is a diuretic herb that is traditionally used to treat diabetes mellitus, a disease having an alarmingly high prevalence rate worldwide. Although various micro molecules present in B. procumbens are known for their antidiabetic roles, the macromolecular constituents have not been studied yet. The current study was therefore planned to identify and characterize the antidiabetic proteins/peptides, if any, present in B. procumbens. Initially the proteins/peptides were extracted, from roots and whole plant of B. procumbens, in sodium phosphate buffers each of concentration 200 mM and of variable pH (range 6-8). The crude extracts were primarily tested for their inhibitory potential against α-glucosidase and α-amylase. The crude extracts with significant inhibitory potential were processed for partial purification of proteins/peptides through the ammonium sulphate (NH<sub>4</sub>)<sub>2</sub>SO<sub>4</sub> precipitation method. The precipitated proteins/peptides were purified through dialysis, and purified fractions, followed by protein quantification, were tested for anti-α-glucosidase and anti-α-amylase activity. The proteins/peptides mixtures were resolved on SDS PAGE, and prominent gel bands were cut and subjected to LC-MS/MS. All crude extracts were found to strongly inhibit both enzymes (i.e., α-glucosidase and anti-α-amylase). Only 03 proteins/peptides fractions were inhibitory to α-glucosidase (with IC<sub>50</sub> range of 137.8 ± 1.2 µg/mL- 352.3 ± 1.2 µg/mL), while 09 fractions were active against α-amylase (with IC<sub>50</sub> range of 24.24 ± 1.8 µg/mL to 85.55 ± 4 µg/mL). A total of 20 proteins were identified via LC-MS/MS analysis, including 04 well-known antidiabetic proteins, i.e., γ-conglutin, thioredoxin peroxidase, 7S globulin, and Zerumbone synthase enzyme. Our study has provided reliable molecular evidence that assures the antidiabetic potential of B. procumbens and suggests the future extension of work for the purification, functional characterization and therapeutic application of antidiabetic proteins.
인공지능 문자 인식 모델을 통해 추출된 텍스트로, 일부 오타나 오류가 포함될 수 있으나 지속적으로 개선 중입니다.
오류를 발견하셨다면 해당 부분을 드래그한 후 ' 를 통해 신고해주세요.
오류를 발견하셨다면 해당 부분을 드래그한 후 ' 를 통해 신고해주세요.
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