인문학
사회과학
자연과학
공학
의약학
농수해양학
예술체육학
복합학
DBpia AI
AI 에이전트
AI 아이디어
AI 리더
AI 뷰어
Citeasy
크롬 서지관리 다운로드
지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
초록·키워드
Lipids are essential for crustacean reproduction, supporting broodstock growth and ovarian development. However, studies of n-3 highly unsaturated fatty acids (HUFAs), particularly eicosapentaenoic acid (EPA), on the growth and ovarian development of prawn broodstock remain limited. Accordingly, five experimental diets containing EPA concentrations of 0.12%, 0.79%, 1.46%, 2.21%, and 2.78% were formulated to examine their effects on ovarian development and broodstock health in the giant freshwater prawn <i>Macrobrachium rosenbergii</i> (initial weight: 9.32 ± 0.52 g) and to determine dietary EPA requirements during ovarian maturation. The results were as follows: (1) no significant differences in survival rate were observed among groups. Weight gain (WG) initially increased and then declined, reaching the highest values in the 1.46% EPA group, although differences among treatments were not significant. In contrast, hepatopancreas index decreased significantly with increasing dietary EPA (<i>p</i> < 0.05). (2) Dietary EPA significantly altered hepatopancreatic fatty acid composition. Saturated fatty acid (SFA) levels showed no significant differences, whereas monounsaturated fatty acids (MUFAs) decreased significantly (<i>p</i> < 0.05). In contrast, polyunsaturated fatty acids (PUFAs) and HUFA increased significantly with higher dietary EPA (<i>p</i> < 0.05), peaking in the 2.78% EPA group. (3) Antioxidant parameters, including total antioxidant capacity (T-AOC), total superoxide dismutase (T-SOD), and glutathione peroxidase (GSH-Px), followed a pattern of initial increase followed by decline with higher EPA levels (<i>p</i> < 0.05). Malondialdehyde (MDA) content showed the opposite trend, reaching its lowest level in the 1.46% EPA group (<i>p</i> < 0.05). (4) Ovarian histology revealed that the 1.46% EPA group exhibited a higher proportion of mature oocytes, with most females reaching ovarian development stages III-IV, and this group also showed the highest gonadosomatic index (GSI). Steroid hormone secretion was significantly affected by dietary EPA (<i>p</i> < 0.05). (5) At the molecular level, EPA inhibited the expression of genes related to lipid synthesis in the hepatopancreas (<i>p</i> < 0.05) and promoted fatty acid <i>β</i>-oxidation, but excessive EPA caused irreversible hepatopancreatic damage. Polynomial regression analysis of steroid hormone secretion indicated that 1.32% and 1.50% dietary EPA supported maximum progesterone (PROG) and 17<i>β</i>-estradiol (E2) levels, respectively. Overall, a dietary EPA level of 1.46% was found to promote broodstock growth, enhance antioxidant capacity, accelerate fatty acid <i>β</i>-oxidation, stimulate steroid hormone secretion, and provide sufficient energy for ovarian development in the giant freshwater prawn.
인공지능 문자 인식 모델을 통해 추출된 텍스트로, 일부 오타나 오류가 포함될 수 있으나 지속적으로 개선 중입니다.
오류를 발견하셨다면 해당 부분을 드래그한 후 ' 를 통해 신고해주세요.
오류를 발견하셨다면 해당 부분을 드래그한 후 ' 를 통해 신고해주세요.
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