인문학
사회과학
자연과학
공학
의약학
농수해양학
예술체육학
복합학
DBpia AI
AI 에이전트
AI 아이디어
AI 리더
AI 뷰어
Citeasy
크롬 서지관리 다운로드
지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
초록·키워드
Native human corneal stromal keratocytes (HCSKs) deposit extracellular matrix (ECM) to maintain corneal homeostasis, repair, and regeneration. Adding macromolecular crowders (MMCs) to culture media encourages HCSKs to produce ECM in vitro. However, MMCs may also be used as serum or growth factor replacement for the in vitro culture of cells. This could control phenotype and reduce the cost of future industrial-scale production of cells such as HCSKs for predicted therapeutic applications in corneal repair. To identify the effects of adding (MMCs) to a serum-free medium and the possible role of MMCs in supporting the growth of HCSKs in an undifferentiated phenotype. Primary HCSKs isolated from human corneas were cultured in serum-free media supplemented with different concentrations of MMCs additive (0%, 4%, and 8%). The influence of the MMCs on proliferation and protein expression was investigated using quantitative colorimetric analysis. The addition of MMCs increased cell proliferation and enhanced expression of quiescent keratocyte markers such as ALDH3A1 and Collagen V. It also downregulated stromal fibroblasts’ specific marker, metalloprotease 2 (MMP2), and α smooth muscle actin. These results indicate that adding MMCs to serum-free media significantly supported cell behaviour. In addition, its presence suggested the retention of native quiescent markers when expanded ex vivo. Moreover, the addition of MMCs to serum-free media enhances human corneal keratocyte proliferation and ECM content, but its effects vary based on media glucose concentration. In standard glucose media, MMCs boost cell proliferation and increase collagen V expression as an indicator for robust ECM; however, there is a higher risk of fibroblastic transformation. In serum-free media, MMCs support a quiescent keratocyte phenotype, reduce metabolic stress, and better mimic the native corneal environment, making it more suitable for regenerative applications.
인공지능 문자 인식 모델을 통해 추출된 텍스트로, 일부 오타나 오류가 포함될 수 있으나 지속적으로 개선 중입니다.
오류를 발견하셨다면 해당 부분을 드래그한 후 ' 를 통해 신고해주세요.
오류를 발견하셨다면 해당 부분을 드래그한 후 ' 를 통해 신고해주세요.
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