목차

1. 서 론 ·································································· 1
1.1. 연구 배경 ······················································ 1
1.2. 문헌 연구 ······················································ 3
1.2.1. 습지 내 질소 생지화학적 순환 특성 ·········· 3
1.2.1.1. 질산화 (Nitrification) ························· 5
1.2.1.2. 탈질화 (Denitrification) ······················ 6
1.2.1.3. DNRA (Dissimilatory nitrate reduction to ammonium) ················································· 8
1.2.2. 기후변화가 습지 내 질소 거동에 미치는 영향 인자 ···················································· 9
1.2.2.1. 식물 생장의 변화 ···························· 11
1.2.1.2. 퇴적토 내 환경 변화 ······················· 13
1.2.1.3. 미생물 군집 특성 변화 ···················· 15
1.2.3. 연구 동향 및 필요성 ······························· 16
1.3. 연구 목표 ······················································ 17
1.3.1. 연구 목적 ·············································· 17
1.3.2. 연구 범위 ·············································· 17
2. 연구 재료 및 방법 ················································ 18
2.1. 연구 지역 및 지점 ·········································· 18
2.2. 습지 지표수 및 지하수위 모니터링 ···················· 21
2.3. 습지 퇴적토 시추조사 ······································ 22
2.4. 습지 심도별 공극수 및 퇴적토 시료 채취 ··········· 24
2.5. 습지 공극수 및 퇴적토 물리화학적 분석 ············ 27
2.6. 실험실 규모의 습지 퇴적토 잠재적 탈질율 측정 ·· 29
2.7. 습지 퇴적토의 미생물 특성 분석 ······················· 34
2.6.1. DNA 추출 및 미생물 군집 분석 ················ 34
2.6.2. qPCR 분석 ············································· 35
2.8. 통계 분석 ······················································ 37
3. 결과 및 고찰 ························································ 38
3.1. 계절에 따른 심도별 이화학적 특성 ···················· 38
3.1.1. 심도별 공극수의 이화학적 특성 ················ 38
3.1.2. 심도별 퇴적토의 이화학적 특성 ················ 43
3.2. 계절에 따른 퇴적토 심도별 질산염 환원 특성 ····· 46
3.2.1. 습지 퇴적토의 잠재적 탈질율 ··················· 46
3.2.2. 습지 퇴적토의 질소 환원 관련 유전자 qPCR 60
3.2.3. 습지 퇴적토의 미생물 군집 분석 ··············· 63
3.3. 계절에 따른 공극수와 퇴적토 질소화합물 인자 사이의 상관성 ················································ 74
3.4. 계절에 따른 잠재적 탈질율과 환경 인자 사이의 상관성 ··························································· 77
3.4.1. 잠재적 탈질율과 환경 인자 변수 사이의 관계 ································································· 77
3.4.2. 잠재적 탈질율과 유전자 및 미생물 특성 사이의 관계 ······················································ 86
4. 결론 ···································································· 89
참고문헌 ······························································ 91