인문학
사회과학
자연과학
공학
의약학
농수해양학
예술체육학
복합학
DBpia AI
AI 에이전트
AI 아이디어
AI 리더
AI 뷰어
Citeasy
크롬 서지관리 다운로드
지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
피인용 0
초록·키워드
Background: Selaginella tamariscina (P. Beauv.) Spring is an important medicinal plant known as ‘Gwonbaek’ in Korea. However, its morphological similarity to S. stauntoniana Spring and S. involvens (Sw.) Spring can lead to misidentification in herbal materials. Therefore, reliable molecular markers are needed to distinguish these species.
Methods and Results: Complete plastome sequences of the three Selaginella species were compared to identify species-specific sequence variations. Phylogenetic analysis based on multiple-sequence alignments resolved the three species into distinct clades. Based on Insertion or Deletion (InDel) regions in the plastomes, four gel-based InDel markers were developed and evaluated using 10 Selaginella samples. The markers distinguished the three species based on their PCR amplification patterns and identified a market sample as S. tamariscina.
Conclusions: The four plastome-based InDel markers showed preliminary discriminatory ability among the tested samples of the three Selaginella species, demonstrating their potential for the molecular identification and quality control of Gwonbaek-related medicinal plant materials.
Methods and Results: Complete plastome sequences of the three Selaginella species were compared to identify species-specific sequence variations. Phylogenetic analysis based on multiple-sequence alignments resolved the three species into distinct clades. Based on Insertion or Deletion (InDel) regions in the plastomes, four gel-based InDel markers were developed and evaluated using 10 Selaginella samples. The markers distinguished the three species based on their PCR amplification patterns and identified a market sample as S. tamariscina.
Conclusions: The four plastome-based InDel markers showed preliminary discriminatory ability among the tested samples of the three Selaginella species, demonstrating their potential for the molecular identification and quality control of Gwonbaek-related medicinal plant materials.
본문·목차
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