인문학
사회과학
자연과학
공학
의약학
농수해양학
예술체육학
복합학
DBpia AI
AI 에이전트
AI 아이디어
AI 리더
AI 뷰어
Citeasy
크롬 서지관리 다운로드
지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2003.2
- 수록면
- 9 - 12 (4page)
이용수
초록· 키워드
The purpose of this study was to evaluate bacteriocin activity against cariogenic bacteria including Streptococcus mutans. The spot-on-lawn method was used to determine the antimicrobial activity against indicator strains. A bacteriocin produced by Lactococcus sp. HY 449 inhibited the growth of S. mutans 3065, S. mutans MT 8148, S. mutans CNU, S. sorbrinus ATCC 27607, S. mitis KCTC 3556, and S. vestibularis KCTC 3650. The bacteriocin was partially purified by ammonium sulfate precipitation and hydrophobic interaction chromatography (HIC). Using chromatography with octyl-sepharose CL-4B, higher molecular weight and undesirable molecules were eluted with ammonium sulfate and H₂O, while the bacteriocin was eluted with ethanol. Purified bacteriocin was added to S. mutans MT 8148 cells during logarithmic growth. After 5 hr of incubation, the optical density (O.D. 660 nm) of cells without bacteriocin increased. Whereas the O.D. of cell suspensions decreased in the presence of bacteriocin, indicating that bacteriocin appears to cause cell lysis of tested strains. The addition of bacteriocin statistically showed decrease of plaque formation (P<0.05). The mean weight of plaque in the culture of S. mutans MT 8148 exhibited 82.8 mg/20 mL of M17-5% sucrose broth, whereas the mean weight of plaque was 11.8 mg/20 mL of M17-5% sucrose broth in the presence of 100 AU/mL bacteriocin. These results suggest that bacteriocin produced by Lactococcus sp. HY 449 is potentially useful in controlling cariogenic bacteria.
상세정보 수정요청해당 페이지 내 제목·저자·목차·페이지정보가 잘못된 경우 알려주세요!
목차
- Abstract
- Introduction
- Materials and Methods
- Results and Discussion
- References
참고문헌
참고문헌 신청최근 본 자료
UCI(KEPA) : I410-ECN-0101-2013-573-001520080
