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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2019.12
- 수록면
- 331 - 338 (8page)
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초록· 키워드
오류제보하기
Human Aichivirus A1 (HuAiV-A1) is a waterborne human pathogenic virus classified as Picornaviridae and Kobuvirus. In this study, we developed a method that can detect about 35 minutes faster with the same detection sensitivity level than the previously reported HuAiV-A1 diagnostic RT-PCR primer. The RT-PCR primer sets developed in this study are capable of detecting HuAiV-A1 at a level of about 100 ag and formed 563 bp amplification product. In addition, the RT-nested PCR method was able to amplify 410 bp using the RT-PCR product as a template. The detection sensitivity of our method was 10 times higher than the method with the highest detection sensitivity to date. Therefore, the detection method of HuAiV-A1 developed in this study is expected to be used in the water environment in which a small amount of virus exists. Also, this detection method is expected to be used as HuAiV-A1 diagnostic technology in both clinical and non-clinical field.
목차
서론
재료 및 방법
결과
고찰
REFERENCES
참고문헌 (16)
참고문헌 신청
Alcalá A / 2010 / Molecular detection and characterization of Aichi viruses in sewage-polluted waters of Venezuela / Appl Environ Microbiol 76:4113~4115
조규봉 / 2018 / Development of Nested PCR Primer Set for the Specific and Highly Sensitive Detection of Human Parvovirus B19 / 대한의생명과학회지 24(4):390~397
Guyader FSL / 2008 / Aichi virus, Norovirus, Astrovirus, Enterovirus, and Rotavirus involved in clinical cases from a french oysterrelated gastroenteritis outbreak / J Clin Microbiol 46:4011~4017
Khamrin P / 2014 / Epidemiology of human and animal kobuviruses / Virus Dis 25:195~200
Khamrin P / 2011 / A single-tube multiplex PCR for rapid detection in feces of 10 viruses causing diarrhea / J Virol Methods 173:390~393
조규봉 / 2018 / Development of Nested PCR Primer Set for the Specific and Highly Sensitive Detection of Human Parvovirus B19 / 대한의생명과학회지 24(4):390~397
Guyader FSL / 2008 / Aichi virus, Norovirus, Astrovirus, Enterovirus, and Rotavirus involved in clinical cases from a french oysterrelated gastroenteritis outbreak / J Clin Microbiol 46:4011~4017
Khamrin P / 2014 / Epidemiology of human and animal kobuviruses / Virus Dis 25:195~200
Khamrin P / 2011 / A single-tube multiplex PCR for rapid detection in feces of 10 viruses causing diarrhea / J Virol Methods 173:390~393
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UCI(KEPA) : I410-ECN-0101-2020-510-000238592