지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2018.12
- 수록면
- 390 - 397 (8page)
이용수
초록· 키워드
오류제보하기
For the specific detection of human Parvovirus B19 (HuPaV-B19), we designed ten specific PCR primers from 3,800~4,500 nucleotides of HuPaV-B19 complete genome (NC_000883.2). Seventeen candidate PCR primer sets for specific detecting HuPaV-B19 were constructed. In specific reaction of HuPaV-B19, seventeen PCR primer sets showed specific band, however five PCR primer sets were selected basis of band intensity, amplicon size and location. In nonspecific reaction with seven reference viruses, four PCR primer sets showed non-specific band, however one PCR primer set is not. Detection sensitivity of final selective PCR primer set was 100 fg/μL for 112 minute, and PCR amplicon is 539 base pairs (bp). In addition, nested PCR primer set was developed, for detection HuPaV-B19 from a low concentration of contaminated samples. Selection of nested PCR primer set was basis of sensitivity and groundwater sample tests. Detection sensitivity of final selective PCR and nested PCR primer sets for the detection of HuPaV-B19 were 100 fg/μL and 100 ag/μL basis of HuPaV-B19 plasmid, it was able to rapid and highly sensitive detection of HuPaV-B19 than previous reports. We expect developed PCR primer set in this study will used for detection of HuPaV-B19 in various samples.
목차
서론
재료 및 방법
결과
고찰
REFERENCES
참고문헌 (23)
참고문헌 신청
[학술지(정기간행물)] - Bergallo M / 2008 / Variants of Parvovirus B19: bioinformatical evaluation of nested PCR assays / Intervirology 51 : 75 ~ 80
[학술지(정기간행물)] - Bock CT / 2014 / Molecular phenotypes of human parvovirus B19 in patients with myocarditis / World J Cardiol 26 : 183 ~ 195
[학술지(정기간행물)] - Bonvicini F / 2009 / Diagnosis of fetal parvovirus B19 infection: value of virological assays in fetal specimens / BJOG 116 : 813 ~ 817
[학술지(정기간행물)] - Bostic JR / 1999 / Quantitative analysis of neutralizing immune responses to human parvovirus B19using a novel reverse transcriptase-polymerase chain reactionbased assay / J Infect Dis 179 : 619 ~ 626
[학술지(정기간행물)] - Brown CS / 1990 / An immunofluorescence assay for the detection of parvovirus B19 IgG and IgM antibodies based on recombinant viral antigen / J Virol Methods 29 : 53 ~ 62
[학술지(정기간행물)] - Bock CT / 2014 / Molecular phenotypes of human parvovirus B19 in patients with myocarditis / World J Cardiol 26 : 183 ~ 195
[학술지(정기간행물)] - Bonvicini F / 2009 / Diagnosis of fetal parvovirus B19 infection: value of virological assays in fetal specimens / BJOG 116 : 813 ~ 817
[학술지(정기간행물)] - Bostic JR / 1999 / Quantitative analysis of neutralizing immune responses to human parvovirus B19using a novel reverse transcriptase-polymerase chain reactionbased assay / J Infect Dis 179 : 619 ~ 626
[학술지(정기간행물)] - Brown CS / 1990 / An immunofluorescence assay for the detection of parvovirus B19 IgG and IgM antibodies based on recombinant viral antigen / J Virol Methods 29 : 53 ~ 62
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UCI(KEPA) : I410-ECN-0101-2019-510-000302767