지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2015.1
- 수록면
- 205 - 210 (6page)
이용수
초록· 키워드
오류제보하기
Prevotella intermedia-specific quantitative real-time PCR (qPCR) primers were previously designed based on the nucleotide sequences of RNA polymerase β-subunit gene (rpoB). However, the several clinical strains isolated from Korean populations are not detectable by the qPCR primers.
The purpose of this study was to develop new P.
intermedia-specific qPCR primers based on the rpoB. The specificity of the primers was determined by conventional PCR with 12 strains of P. intermedia and 52 strains (52 species) of non-P. intermedia bacteria. The sensitivity of primers was determined by qPCR with serial dilutions of the purified genomic DNAs (40 ng to 4 fg) of P. intermedia ATCC 25611T. The data indicated that only P. intermedia strains were detected by the P intermedia-specific qPCR primers (RTPiF2/RTPiR2); in addition, as little as 40 fg of P.
intermedia genomic DNA could be detected. These results suggest that these qPCR primers are useful in detecting P.
intermedia from the bacterial infectious lesions including dental plaque and oral tissue lesions.
목차
등록된 정보가 없습니다.
참고문헌 (17)
참고문헌 신청
Dorn BR / 1998 / Invasion of human oral epithelial cells by Prevotella intermedia / Infect Immun 66 : 6054 ~ 6057
Hiroshi Maeda / 2003 / Quantitative real-time PCR using TaqMan and SYBR Green for Actinobacillus actinomycetemcomitans, Porphyromonas gingivalis, Prevotella intermedia, tetQ
Hwan Seon Shin / 2010 / Development of strain-specific PCR primers for the identification of Fusobacterium nucleatum subsp. fusiforme ATCC 51190T and subsp. vincentii ATCC 4
Joong-Ki Kook / 2005 / Detection of Tannerella forsythia and/or Prevotella intermedia might be useful for microbial predictive markers for the outcome of initial periodontal
Kaoru Saito / 2001 / Effects of glucose on formation of cytotoxic end-products and proteolytic activity of Prevotella intermedia, Prevotella nigrescens and Porphyromonas ging
Hiroshi Maeda / 2003 / Quantitative real-time PCR using TaqMan and SYBR Green for Actinobacillus actinomycetemcomitans, Porphyromonas gingivalis, Prevotella intermedia, tetQ
Hwan Seon Shin / 2010 / Development of strain-specific PCR primers for the identification of Fusobacterium nucleatum subsp. fusiforme ATCC 51190T and subsp. vincentii ATCC 4
Joong-Ki Kook / 2005 / Detection of Tannerella forsythia and/or Prevotella intermedia might be useful for microbial predictive markers for the outcome of initial periodontal
Kaoru Saito / 2001 / Effects of glucose on formation of cytotoxic end-products and proteolytic activity of Prevotella intermedia, Prevotella nigrescens and Porphyromonas ging
함께 읽어보면 좋을 논문
논문 유사도에 따라 DBpia 가 추천하는 논문입니다. 함께 보면 좋을 연관 논문을 확인해보세요!
-
Development of species-specific quantitative real-time PCR primers for detecting Lautropia mirabilis
International Journal of Oral Biology
2021 .09
-
Identification and characterization of a hemagglutinin gene from Prevotella intermedia
Oral Biology Research
2021 .01
-
Development of Quantitative Real-Time PCR Primers for Detection of Streptococcus sobrinus
International Journal of Oral Biology
2016 .01
-
Peptoniphilus mikwangii -specific quantitative real-time polymerase chain reaction primers
International Journal of Oral Biology
2019 .01
-
일부 대학생들의 구강 내 치주질환 세균 분포와 검출 위험요인 조사
한국치위생학회지
2017 .01
-
Simultaneous Analysis of Four Standards of The Herbal Formula, DF-02, of Ephedra intermedia and Rheum palmatum, using by High Performance Liquid Chromatography-Ultraviolet Detector (HPLC-UVD)
Natural Product Sciences
2019 .06
-
Tetra Primer ARMS PCR Optimization to Detect Single Nucleotide Polymorphisms of the CYP2E1 Gene
Asian Pacific journal of cancer prevention : APJCP
2016 .01
-
Development of Nested PCR Primer Set for the Specific and Highly Sensitive Detection of Human Parvovirus B19
대한의생명과학회지
2018 .12
-
Comparison of Primers for Oral Mycobiome Study in Intubated Patients
JOURNAL OF BACTERIOLOGY AND VIROLOGY
2021 .09
-
Development of Species-specific PCR Primers for Detecting Peptoniphilus mikwangii
International Journal of Oral Biology
2017 .01
-
A Rapid and Sensitive Detection of Aflatoxin-producing Fungus Using an Optimized Polymerase Chain Reaction (PCR)
Toxicological Research
2016 .01
-
Primer가 이중중합 레진시멘트의 중합에 미치는 영향
대한치과의사협회지
2020 .11
-
Changes in Oral Microbial Population through Dental Scaling and Dental Polishing
International Journal of Clinical Preventive Dentistry
2019 .01
-
타액내 구강질환 원인 균의 세균배양법, SYBR green qPCR법, MRT-PCR법 간의 정량분석
한국치위생학회지
2017 .01
-
qPCR을 이용한 미생물 유전자 정량 분석에 대한 마그네슘 이온 기반 환경 저해 인자의 영향
환경독성보건학회 심포지엄 및 학술대회
2022 .10
-
Study of Oral Microbial Prevalence and Oral Health in Adults
International Journal of Clinical Preventive Dentistry
2018 .01
-
Construction of Improved PCR Primer Set for the Detection of Human Enteric Adenovirus 41
대한의생명과학회지
2018 .09
-
Primer를 함께 사용하는 자가미백 부착대의 임상적 효과
대한구강보건학회지
2016 .03
-
Decaschistia intermedia Craib 추출물의 멜라닌 합성 억제 효능
생약학회지(Korean Journal of Pharmacognosy)
2020 .09
-
약전에 수재된 기원 3종의 마황에 대한 외부형태 감별기준
한약정보연구회지
2016 .11
이 논문의 저자 정보
최근 본 자료
댓글(0)
0