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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2016.1
- 수록면
- 149 - 154 (6page)
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초록· 키워드
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The purpose of this study was to develop Streptococcus sobrinus-specific qPCR primers based on the nucleotide sequence of the RNA polymerase β-subunit gene (rpoB). The specificity of the primers was determined by conventional polymerase chain reaction (PCR) with 12 strains of S.
sobrinus and 50 strains (50 species) of non-S. sobrinus bacteria. The sensitivity of the primers was determined by quantitative real-time PCR (qPCR) with serial dilutions of the purified genomic DNAs (40 ng to 4 fg) of S. sobrinus ATCC 33478T. The specificity data showed that the S.
sobrinus-specific qPCR primers (RTSsob-F4/RTSsob-R4) detected only the genomic DNAs of S. sobrinus strains with a detection limit of up to 4 fg of S. sobrinus genomic DNA.
Our results suggest that the RTSsob-F4/RTSsob-R4 primers are useful in detecting S. sobrinus with high sensitivity and specificity for epidemiological studies of dental caries..
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등록된 정보가 없습니다.
참고문헌 (12)
참고문헌 신청
Loesche WJ / 1986 / Role of Streptococcus mutans in human dental decay / Microbiol Rev 50:353~380
Aas JA / 2005 / Defining the normal bacterial flora of the oral cavity / J Clin Microbiol 43:5721~5732
Whiley RA / 1998 / Current classification of the oral streptococci / Oral Microbiol Immunol 13:195~216
Soon-Nang Park / 2013 / Development of quantitative real-time PCR primers for detecting 42 oral bacterial species / Archives of Microbiology 195(7):473~482
Wilson K / 1990 / Current Protocols in Molecular Biology / Greene Publ. Assoc. and Wiley Interscience :241~245
Aas JA / 2005 / Defining the normal bacterial flora of the oral cavity / J Clin Microbiol 43:5721~5732
Whiley RA / 1998 / Current classification of the oral streptococci / Oral Microbiol Immunol 13:195~216
Soon-Nang Park / 2013 / Development of quantitative real-time PCR primers for detecting 42 oral bacterial species / Archives of Microbiology 195(7):473~482
Wilson K / 1990 / Current Protocols in Molecular Biology / Greene Publ. Assoc. and Wiley Interscience :241~245
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