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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2021.12
- 수록면
- 155 - 159 (5page)
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초록· 키워드
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This study aimed to develop strain-specific polymerase chain reaction (PCR) primers to detect Fusobacterium hwasookii KCOM 1249T, F. hwasookii KCOM 1253, F. hwasookii KCOM 1256, F. hwasookii KCOM 1258, and F.
hwasookii KCOM 1268 on the basis of nucleotide sequences of a gene specific to each strain. The unique genes for each F. hwasookii strain were determined on the basis of their genome sequences using Roary. The strain-specific PCR primers based on each strain-specific gene were designed using PrimerSelect. The specificity of each PCR primer was determined using the genomic DNA of the 5 F. hwasookii strains and 25 strains of oral bacterial species.
The detection limit and sensitivity of each strain-specific PCR primer pair were determined using the genomic DNA of each target strain. The results showed that the strain-specific PCR primers correspond to F. hwasookii KCOM 1249T, F. hwasookii KCOM 1253, F. hwasookii KCOM 1258, F. hwasookii KCOM 1256/F. nucleatum subsp. polymorphum KCOM 1260, or F. hwasookii KCOM 1268/Fusobacterium sp. oral taxon 203 were developed. The detection limits of these strain-specific PCR primers ranged from 0.2 to 2 ng of genomic DNA for each target strain. The results suggest that these strain-specific PCR primers are valuable in quality control for detecting specific F. hwasookii strains.
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참고문헌 (10)
참고문헌 신청
Cho E / 2015 / Fusobacterium hwasookii sp. nov., isolated from a human periodontitis lesion / Curr Microbiol 70:169~175
Krieg NR / 2001 / Bergey’s manual of systematic bacteriology, Vol. 1 / Springer :33~38
Chun J / 2018 / Proposed minimal standards for the use of genome data for the taxonomy of prokaryotes / Int J Syst Evol Microbiol 68:461~466
Lee I / 2016 / OrthoANI : an improved algorithm and software for calculating average nucleotide identity / Int J Syst Evol Microbiol 66:1100~1103
Hwa-Sook Kim / 2005 / Development of Strain-specific PCR Primers Based on a DNA Probe Fu12 for the Identification of Fusobacterium nucleatum subsp. nucleatum ATCC 25586T / The Journal of Microbiology 43(4):331~336
Krieg NR / 2001 / Bergey’s manual of systematic bacteriology, Vol. 1 / Springer :33~38
Chun J / 2018 / Proposed minimal standards for the use of genome data for the taxonomy of prokaryotes / Int J Syst Evol Microbiol 68:461~466
Lee I / 2016 / OrthoANI : an improved algorithm and software for calculating average nucleotide identity / Int J Syst Evol Microbiol 66:1100~1103
Hwa-Sook Kim / 2005 / Development of Strain-specific PCR Primers Based on a DNA Probe Fu12 for the Identification of Fusobacterium nucleatum subsp. nucleatum ATCC 25586T / The Journal of Microbiology 43(4):331~336
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