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자료유형
학술저널
저자정보
저널정보
한국식품영양과학회 Journal of Food Science and Nutrition Journal of Food Science and Nutrition Vol.5 No.4
발행연도
2000.12
수록면
230 - 233 (4page)

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The present work was designed to determine whether change in fluidity of the mitochondrial membrane affects mitochondrial F₁FoATPase characteristics in NIDDM-prone BHE/Cdb rat. Isolated mitochondria from BHE/Cdb rat fed a 6% coconut oil or corn oil were functionally tested by an analysis of its respiration and the coupling of this process to ATP synthesis in presence of oligomycin, a specific inhibitor of oxidative phosphorylation (OXPHOS), that binds to the F₁FoATPase. Mitochondria from rats fed coconut oil were more responsive to the inhibitory action of oligomycin with respect to state 3 respiration, respiratory control (RC) ratio and ADP:P (P/O) ratio than were mitochondria from rats fed corn oil. In state 3 respiration, mitochondria from rats fed coconut oil consumed less oxygen than did mitochondria from rats fed corn oil. RC ratio was lower in the mitochondria from rats fed coconut oil than was mitochondria from rats fed corn oil. In P/O ratio, the mitochondria from rats fed coconut oil had a lower P/O ratio than did mitochondria from rats fed corn oil. The data showed that the change in fluidity of the mitochondrial membrane by dietary fat affected mitochondrial F₁FoATPase characteristics. The present study on diet differences in F₁FoATPase characteristics provides considerable insight into the role diets play in the control of mitochondrial function, especially OXPHOS in NIDDM with mitochondrial defects.

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Abstract

INTRODUCTION

MATERIALS AND METHODS

RESULTS AND DISCUSSION

ACKNOWLEDGEMENTS

REFERENCES

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