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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
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A cDNA encoding the masu salmon, Oncorhynchus masou, estrogen receptor α (msERα) was cloned from the pituitary gland by polymerase chain reaction (PCR). This cDNA contains an open reading frame encoding 513 amino acid residues, and the calculated molecular weight of this protein is about 56,430 Dalton. The amino acid sequences of the DNA binding and ligand binding domains of msERα showed high homology to those of other fish species (84-100%). Reverse transcription PCR analysis showed that the mRNA level of msERα in the pituitary was slightly higher in estradiol-17β(E2) injected masu salmon than that of control fish. To test the biological activity of msERα, the cDNA was ligated to a mammalian expression vector and transfected into a gonadotropederived cell line, LβT2, with a reporter plasmid including estrogen responsive element. Expression of the reporter protein, luciferase, was E2 and msERα- dependent. The masu salmon ERα is structurally conserved among teleost species and functions as a transcriptional activator in the pituitary cells.
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Abstract
Introduction
Material and Methods
Results and Discussion
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UCI(KEPA) : I410-ECN-0101-2009-529-015356268