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학술저널
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한국원예학회 HORTICULTURE ENVIRONMENT and BIOTECHNOLOGY HORTICULTURE ENVIRONMENT and BIOTECHNOLOGY Vol.47 No.5
발행연도
2006.10
수록면
280 - 287 (8page)

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Effects of salicylic acid (SA) were investigated on cuttings of ‘Kyoho’ grapevine cultivar exposed to low temperature to investigate effects of SA on chilling stress. The chilling condition was 5℃ in the dark for 12 h and additive illumination of 100 μ㏖ㆍm?²ㆍs?¹ PPF for 0, 1, 2, and 3 h. One day before chilling, the cuttings were pretreated by spraying 0, 0.5, 1.0, 1.5, and 2.0 mM SA hydroponic solution onto the leaves. Under low temperature, young leaves showed lower photo system Ⅱ (PSⅡ) activities than mature leaves, but SA at 1.5 mM alleviated the decrease of photosynthetic ability. Ascorbate peroxidase (APX) activities at 25℃ were maintained as steady state, but increased at 5℃ about 200% compared with that of 25℃ at 2 h after the illumination. Catalase (CAT) activities also increased during 2 h illumination, but the similar change with less magnitude was observed at 5℃. Under low temperature, activities of peroxidase (POD) and glutathione reductase (GR) increased 3 h after illumination. Decreased H₂O₂ level at 12/2 h (dark/light) and increased H₂O₂ level at 12/3 h imply that APX and CAT would be key enzymes to remove H₂O₂’ SA increased APX activities at both 25 and 5℃. In addition, APX activities increased at 12 and 24 h, prior to chilling stress, and after SA treatment. POD also showed higher activities at 12 and 24 h after SA treatment and GR activities increased at 12 h. CAT activities were inhibited or maintained by SA under normal and low temperature. Although activities of total antioxidants decreased at 25℃ by SA, the activities were higher than those without SA spray, and increased according to time under low temperature. Higher APX, POD, and GR activities prior to chilling stress and activities of total antioxidants during chilling stress caused low H₂O₂ level in SA treated leaves. This may be a factor to alleviate reduction of PSI! activities under low temperature. Furthermore, SA spray at appropriate concentration could be used as an alleviator against chilling.

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Abstract
Introduction
Materials and Methods
Results and Discussion
Acknowledgement
Literature Cited

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