지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2008.6
- 수록면
- 114 - 119 (6page)
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초록· 키워드
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Terpene synthase plays a key role in biosynthesis of triterpene saponins (ginsenosides) and is intermediate in the biosynthesis of a number of secondary metabolites. A terpene synthase (PgTPS) cDNA was isolated and characterized from the root of Panax ginseng C.A. Meyer. The deduced amino acid sequence of PgTPS showed a similarity with A. deliciosa (AAX16121) 61%, V. vinifera (AAS66357) 61%, L hirsutum (AAG41891) 55%, M truncatula (AAV36464) 52%. And the segment of a terpene synthase gene was amplified by reverse transcriptase-polymerase chain reaction (RT-PCR). We studied expression of terpene synthase under stressful conditions like chilling, salt, UV, and heavy metal stress treatment. Expression of PgTPS was increased gradually after exposure to stresses such as chilling, salt, and UV illumination. But its transcription seems to be reduced by cadmium and copper treatment.
목차
Abstract
INTRODUCTION
MATERIALS AND METHODS
RESULTS
DISCUSSION
ACKNOWLEDGEMENTS
REFERENCES
참고문헌 (26)
참고문헌 신청
Croteau, R. , 2000 , Natural Products (Secondary Metabolites). Biochemistry and molecular biology of plants , American Society of plant Biologists : 1250 ~ 1318
Bouwmeester, H. J. , 2002 , Isolation and characterization of two germacrene a synthase cDNA clones from chicory , Plant Physiol 129 : 134 ~ 144
Kushiro, T. , 1997 , In vitro conversion of 2,3-oxidosqualene into dammarenediol by panax ginseng microsomes , Biol. Pharm. Bull 20 : 292 ~ 294
Bohlmann, J. , 1998 , Terpenoid-based defenses in conifers: cDNA cloning, characterization,and functional expression of wound-inducible (E)-α-bisabolene synthase from grand fir (Abies grandis) , Proc.Natl. Acad. Sci. USA 95 : 6756 ~ 6761
Lee, M. H. , 2004 , Enhanced triterpene and phytosterol biosynthesis in Panax ginseng overexpressing squalene synthase hene , Plant Cell Physiol 45 : 976 ~ 984
Bouwmeester, H. J. , 2002 , Isolation and characterization of two germacrene a synthase cDNA clones from chicory , Plant Physiol 129 : 134 ~ 144
Kushiro, T. , 1997 , In vitro conversion of 2,3-oxidosqualene into dammarenediol by panax ginseng microsomes , Biol. Pharm. Bull 20 : 292 ~ 294
Bohlmann, J. , 1998 , Terpenoid-based defenses in conifers: cDNA cloning, characterization,and functional expression of wound-inducible (E)-α-bisabolene synthase from grand fir (Abies grandis) , Proc.Natl. Acad. Sci. USA 95 : 6756 ~ 6761
Lee, M. H. , 2004 , Enhanced triterpene and phytosterol biosynthesis in Panax ginseng overexpressing squalene synthase hene , Plant Cell Physiol 45 : 976 ~ 984
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UCI(KEPA) : I410-ECN-0101-2009-524-014846131