지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2010.3
- 수록면
- 17 - 22 (6page)
이용수
이 논문의 연구 히스토리 (3)
2010
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초록· 키워드
오류제보하기
Sesquiterpenes are found naturally in plants and insects as defensive agents or pheromones. They are produced in the cytosolic acetate/mevalonate pathway for isoprenoid biosynthesis. The inducible sesquiterpene synthases (STS), which are responsible for the transformation of the precursor farnesyl diphosphate, appear to generate very few olefinic products that are converted to biologically active metabolites. In this study, we isolated the STS gene from Panax ginseng C.A. Meyer, designated PgSTS, and investigated the correlation between its expression and various abiotic stresses using real-time PCR. PgSTS cDNA was observed to be 1,883 nucleotides long with an open reading frame of 1,707 bp, encoding a protein of 568 amino acids. The molecular mass of the mature protein was determined to be 65.5 kDa, with a predicted isoelectric point of 5.98. A GenBank BlastX search revealed the deduced amino acid sequence of PgSTS to be homologous to STS from other plants, with the highest similarity to an STS from Lycopersicon hirsutum (55% identity, 51% similarity). Real-time PCR analysis showed that different abiotic stresses triggered significant induction of PgSTS expression at different time points.
목차
Abstract
INTRODUCTION
MATERIALS AND METHODS
RESULTS AND DISCUSSION
ACKNOWLEDGMENTS
REFERENCES
참고문헌 (25)
참고문헌 신청
1. [학술지(정기간행물)] - Yu F / 2008 / Isolation and functional characterization of a β-eudesmol synthase, a new sesquiterpene synthase from Zingiber zerumbet Smith / FEBS Letters 582 : 565 ~ 572
2. [학술지(정기간행물)] - Pechous SW / 2004 / Cloning and functional expression of an (E,E)-α-farnesene synthase cDNA from peel tissue of apple fruit / Planta 219 : 84 ~ 94
3. [학술지(정기간행물)] - Cheng AX / 2007 / The rice (E)-β-caryophyllene synthase (OsTPS3) accounts for the major inducible volatile sesquiterpenes / Phytochem 68 : 1632 ~ 1641
4. [학술지(정기간행물)] - Arimura GI / 2008 / Herbivore-induced terpenoid emission in Medicago truncatula: concerted action of jasmonate, ethylene and calcium signaling / Planta 227 : 453 ~ 464
5. [학술지(정기간행물)] - Picaud S / 2005 / Expression, purification, and characterization of recombinant amorpha- 4,11-diene synthase from Artemisia annua L. / Arch Biochem Biophys 436 : 215 ~ 226
2. [학술지(정기간행물)] - Pechous SW / 2004 / Cloning and functional expression of an (E,E)-α-farnesene synthase cDNA from peel tissue of apple fruit / Planta 219 : 84 ~ 94
3. [학술지(정기간행물)] - Cheng AX / 2007 / The rice (E)-β-caryophyllene synthase (OsTPS3) accounts for the major inducible volatile sesquiterpenes / Phytochem 68 : 1632 ~ 1641
4. [학술지(정기간행물)] - Arimura GI / 2008 / Herbivore-induced terpenoid emission in Medicago truncatula: concerted action of jasmonate, ethylene and calcium signaling / Planta 227 : 453 ~ 464
5. [학술지(정기간행물)] - Picaud S / 2005 / Expression, purification, and characterization of recombinant amorpha- 4,11-diene synthase from Artemisia annua L. / Arch Biochem Biophys 436 : 215 ~ 226
함께 읽어보면 좋을 논문
논문 유사도에 따라 DBpia 가 추천하는 논문입니다. 함께 보면 좋을 연관 논문을 확인해보세요!
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Expression Analysis of Sesquiterpene Synthase Gene from Panax ginseng C. A. Meyer
고려인삼학회 학술대회
2010 .12
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Isolation and Characterization of Sesquiterpene Synthase Gene from Panax ginseng C. A. Meyer
고려인삼학회 학술대회
2009 .05
-
인삼 Sesquiterpene의 새로운 분리방법 ( New Methods for Isolation of Sesquiterpene from Panax ginseng )
Journal of Ginseng Research
1997 .12
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Transcript profiling of antioxidant genes during biotic and abiotic stresses in Panax ginseng C. A. Meyer
고려인삼학회 학술대회
2011 .04
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한국인삼과 북미산 서양삼의 Sesquiterpene ( Comparison of Sesquiterpenes in Korean and American Ginsengs )
Journal of Ginseng Research
1997 .12
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Molecular Characterization of Alcohol Dehydrogenase Gene Related to Abiotic Stress from Panax ginseng
고려인삼학회 학술대회
2008 .12
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Isolation and Characterization of Spermidine Synthase Gene from Panax ginseng C. A. Meyer
고려인삼학회 학술대회
2009 .12
-
Isolation and Characterization of Spermidine Synthase Gene from Panax ginseng C. A. Meyer
고려인삼학회 학술대회
2009 .05
-
Breeding of Korean Ginseng ( Panax ginseng C. A. Meyer )
International Symposium on Ginseng
1998 .09
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Isolation and Characterization of Calmodulin Gene from Panax ginseng C. A. Meyer
Journal of Ginseng Research
2009 .03
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Isolation and Characterization of Terpene Synthase Gene from Panax ginseng
Journal of Ginseng Research
2008 .06
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Study on the Response of Korean Ginseng ( Panax ginseng C. A. Meyer ) to the Herbicide 2,4-D Application
International Symposium on Ginseng
1990 .07
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고려인삼 ( Panax ginseng C. A. Meyer ) 의 엽록체 DNA 분리 및 특성조사 ( Isolation and Characterization of Chloroplast DNA in Korean Ginseng , Panax ginseng C. A. Meyer )
Journal of Ginseng Research
1993 .04
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Isolation and Characterization of Cinnamoyl-CoA Reductase Gene from Panax ginseng C. A. Meyer
Journal of Ginseng Research
2008 .09
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Isolation and Characterization of Glycolate Oxidase Gene from Panax ginseng C. A. Meyer
Journal of Ginseng Research
2009 .12
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The Characterization of Mitochondrial DNA of Korean Ginseng ( Panax ginseng C. A. Meyer )
International Symposium on Ginseng
1990 .07
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Isolation and Characterization of a Sucrose Synthase (SUS) Gene from Panax ginseng
고려인삼학회 학술대회
2009 .05
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Isolation of Ribonuclease2 gene from cDNA of Panax ginseng C.A. Meyer
고려인삼학회 학술대회
2008 .12
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Isolation of a novel NADP-Isocitrate Dehydrogenase Gene from Panax ginseng and Analysis of Its Response to Abiotic Stress
고려인삼학회 학술대회
2008 .12
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Isolation and Characterization of a Type Ⅱ Peroxiredoxin Gene from Panax ginseng C. A. Meyer
Journal of Ginseng Research
2010 .12
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UCI(KEPA) : I410-ECN-0101-2010-524-003375724