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PCR performance of different primer sets that are used for detecting Acidovorax avenae subsp. citrulli from bacterial fruit blotch (BFB)-infected plant tissue and seeds in watermelon were evaluated. PCR specificity and sensitivity in detecting Acidovorax avenae subsp. citrulli were examined for four BFB-PCR primer sets. From screening of 10 different bacterial species and three subspecies of Acidovorax avenae, the best PCR-specificity to A. avenae subsp. citrulli was observed from RST 49/51 after digestion of the PCR products with Sau 96I. PCR sensitivity was evaluated by using serial dilutions of template DNA samples achieved from BFB-infected watermelon seeds and leaf tissues, and the highest detection sensitivity was observed from Seminis WFB 1/2. To improve PCR specificity to A. avenae subsp. citrulli of Seminis WFB 1/2, PCR amplicons from five bacterial species were sequenced and a new CAPS marker was developed based on the restriction enzyme site polymorphisms unique to A. avenae subsp. citrulli. CAPS marker uses primer sequence of Seminis WFB 1/2 of high detection sensitivity, and discriminates A. avenae subsp. citrulli from any other bacterial species.

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Abstract
Introduction
Materials and Methods
Results
Discussion
Acknowledgements
Literature Cited

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