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논문 기본 정보

저자정보
(건국대학교) (건국대학교) (건국대학교) (건국대학교) (건국대학교)
저널정보
한국식품과학회 Food Science and Biotechnology Food Science and Biotechnology vol 18. no 4
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    초록·키워드

    Production of the antioxidant substances by Bacillus polyfermenticus SCD was investigated using shake-flask fermentation. The one-factor-at-a-time method was first employed to determine the key ingredients for optimal medium composition, then further investigation of the medium composition was performed using response surface methodology (RSM). The antioxidant activity was measured using 2,2-diphenyl-1-picryl-hydrazyl-hydrate (DPPH) assays. After screening various elements, fructose, tryptone, and MgSO₄ㆍ7H₂O were chosen as the main factors for study in the statistical experimental design. Central composite design (CCD) was then used to determine the optimal concentrations of these 3 components. Under the proposed optimized medium containing 2.8% fructose, 1.34% tryptone, 0.015% MgSO₄ㆍ7H₂O, 0.5% NaCl, and 0.25% K₂HPO₄, the model predicted an antioxidant activity of 80.5% (R²=0.9421). The actual experimental results were in agreement with the prediction.

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      UCI(KEPA) : I410-ECN-0101-2013-573-000874789