지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2011.10
- 수록면
- 235 - 241 (7page)
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Purpose: This study demonstrated that apoptosis induced by mycophenolic acid (MPA) is mediated by mitochondrial membrane potential transition (MPT) changes in Jurkat cells. Methods: Cell viability and MPT changes were measured by flow cytometry. Western blotting was performed to evaluate the expression of Bcl-2 family proteins, Bid, truncated Bid (tBid), cytochrome c, voltage dependent anion channel (VDAC), poly ADP-ribose polymerase (PARP), and protein kinase C-δ (PKC-δ). The catalytic activity of caspase-9 and -3 was also measured. Results: Cell viability was decreased in time- and dose-dependent manners. Bcl-2 protein expression was decreased, but Bax protein expression was identified. A decreased Bcl-XL /Bcl-XS ratio was also noted. The expression of tBid protein also increased in a time-dependent manner in Jurkat cells treated with MPA. While normal MPT appeared as orange fluorescence, abnormal MPT corresponded to green fluorescence. Green fluorescence increased as orange decreased in the MPA-treated cells. Significantly increased concentrations of MPA induced the release of cytosolic cytochrome c. MPA also augmented the catalytic activity of caspase-9 and caspase-3 in Jurkat cells. Our findings demonstrated that MPA-induced apoptosis is mediated by MPT changes accompanied by decreased Bcl-XL expression and the appearance of tBid protein. The release of cytosolic cytochrome c from mitochondria and increased catalytic activity of caspase-9 and caspase-3 were observed in MPA-treated Jurkat cells. Conclusion: These results suggest that mitochondrial dysfunction caused by MPA induces human T lymphocyte apoptosis.
목차
Purpose
INTRODUCTION
METHODS
RESULTS
DISCUSSION
CONFLICTS OF INTEREST
ACKNOWLEDGEMENTS
REFERENCES
참고문헌 (19)
참고문헌 신청
1. [학술지(정기간행물)] - Gross A / 1999 / BCL-2 family members and the mitochondria in apoptosis / Genes Dev 13 : 1899 ~ 1911
2. [학술지(정기간행물)] - Hockenbery D / 1990 / Bcl-2 is an inner mitochondrial membrane protein that blocks programmed cell death / Nature 348 : 334 ~ 336
3. [학술지(정기간행물)] - Crompton M / 2000 / Bax, Bid and the permeabilization of the mitochondrial outer membrane in apoptosis / Curr Opin Cell Biol 12 : 414 ~ 419
4. [학술지(정기간행물)] - Lee WA / 1990 / Bioavailability improvement of mycophenolic acid through amino ester derivatization / Pharm Res 7 : 161 ~ 166
5. [학술지(정기간행물)] - Schulze-Osthoff K / 1998 / Apoptosis signaling by death receptors / Eur J Biochem 254 : 439 ~ 459
2. [학술지(정기간행물)] - Hockenbery D / 1990 / Bcl-2 is an inner mitochondrial membrane protein that blocks programmed cell death / Nature 348 : 334 ~ 336
3. [학술지(정기간행물)] - Crompton M / 2000 / Bax, Bid and the permeabilization of the mitochondrial outer membrane in apoptosis / Curr Opin Cell Biol 12 : 414 ~ 419
4. [학술지(정기간행물)] - Lee WA / 1990 / Bioavailability improvement of mycophenolic acid through amino ester derivatization / Pharm Res 7 : 161 ~ 166
5. [학술지(정기간행물)] - Schulze-Osthoff K / 1998 / Apoptosis signaling by death receptors / Eur J Biochem 254 : 439 ~ 459
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UCI(KEPA) : I410-ECN-0101-2013-514-002683709