The purpose of this study was to investigate the effects of combined(aerobic and resistance) exercise on body composition, health related fitness and GLP-1 in middle aged women with obesity. A total of 20 obese middle-aged women (>25㎏/㎡) were divided into control (n=10) and exercise (n=10) groups. The 12-week exercise intervention consisted of 3 days of combined aerobic and resistance exercise per week. Each go-minute exercise. programme included 10 minutes of warm-up and 10 minutes of cool-down. Blood samples were collected at baseline and immediately after 12 weeks. The results of this study showed that combined exercise improves body composition, cardiorespiratory fitness & health related fitness, serum lipid profile, adiponectin and GLP-1 in obese middle-aged women. Therefore, these data demonstrated that combined exercise programme may represent an effective intervention strategy for improving insulin resistance and cardiovascular disease risk markers in obese middle-aged women.