지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
초록· 키워드
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Background and Objective: Coptidis Rhizoma is a medicinal herb known for its antioxidant and anti-inflammatory effect. The purpose of this study was to examine the effects of CR on the experimental burn elicitation in vitro and in vivo.
Material and Methods: In order to know the antioxidant effect on skin cell of mice after burn elicitation, superoxide dismutase (SOD) activity was measured.
In vitro, the RAW 264.7 macrophage cells were treated with lipopolysaccharides for experimental inflammation. iNOS mRNA expression was observed after CR-treatment. In order to know effects on the skin regeneration in the burned mice, we counted the nitric oxide (NO) in blood. We also observed the histological structure in the epidermal basal layer and the dermal section, and we studied changes of angiogenesis in the capillaries surrounding the basal layer and dermal papilla. The changes of transcription of iNOS mRNA (inducible nitric oxide synthase mRNA) and changes of NF-κB (nuclear factor κB) p65 positive reaction were also observed to investigate the changes of the stress in the skin.
Results: The results indicated that CR has significant effects on the antioxidant effect on skin cells of mice after burn elicitation by increasing SOD activity in the in vitro test. It seemed that CR decreased the amount of NF-kB which induced the iNOS mRNA dose-dependently and suppress activating NO and angiogenesis. Furthermore, CR facilitated the process of skin recovery after experimental burn.
Conclusion: CR can be applied for burned skin via antioxidant effect and skin regeneration.
Material and Methods: In order to know the antioxidant effect on skin cell of mice after burn elicitation, superoxide dismutase (SOD) activity was measured.
In vitro, the RAW 264.7 macrophage cells were treated with lipopolysaccharides for experimental inflammation. iNOS mRNA expression was observed after CR-treatment. In order to know effects on the skin regeneration in the burned mice, we counted the nitric oxide (NO) in blood. We also observed the histological structure in the epidermal basal layer and the dermal section, and we studied changes of angiogenesis in the capillaries surrounding the basal layer and dermal papilla. The changes of transcription of iNOS mRNA (inducible nitric oxide synthase mRNA) and changes of NF-κB (nuclear factor κB) p65 positive reaction were also observed to investigate the changes of the stress in the skin.
Results: The results indicated that CR has significant effects on the antioxidant effect on skin cells of mice after burn elicitation by increasing SOD activity in the in vitro test. It seemed that CR decreased the amount of NF-kB which induced the iNOS mRNA dose-dependently and suppress activating NO and angiogenesis. Furthermore, CR facilitated the process of skin recovery after experimental burn.
Conclusion: CR can be applied for burned skin via antioxidant effect and skin regeneration.
목차
서론
실험 재료 및 방법
실험 결과
고찰
결론
참고문헌
참고문헌 (47)
참고문헌 신청
1. [학술지(정기간행물)] - Brown TT / 2009 / The epidemiology of sunburn in the US population in 2006 / J Am Acad Dermatol 55 (4) : 577 ~ 583
2. [단행본] - 許戟創 / 1997 / 中醫外科傷科名著集成(外科症治全書) / 華夏出版社 : 271 ~
3. [학술지(정기간행물)] - MK Yu / 2003 / A literature review on external treatment of a burn / The Journal of Korean Oriental Medical Ophthalmology and Otolaryngology and Dermatology 16 (3) : 38 ~ 67
4. [단행본] - SB Kim / 2001 / Effect of skin application with Rhubarb, Olibanum and Myrrha / Dep. of Oriental medical graduate school, Dongguk Univ
5. [단행본] - 吳謙 / 1991 / 醫宗金鑑 / 대성문화사 : 448 ~
2. [단행본] - 許戟創 / 1997 / 中醫外科傷科名著集成(外科症治全書) / 華夏出版社 : 271 ~
3. [학술지(정기간행물)] - MK Yu / 2003 / A literature review on external treatment of a burn / The Journal of Korean Oriental Medical Ophthalmology and Otolaryngology and Dermatology 16 (3) : 38 ~ 67
4. [단행본] - SB Kim / 2001 / Effect of skin application with Rhubarb, Olibanum and Myrrha / Dep. of Oriental medical graduate school, Dongguk Univ
5. [단행본] - 吳謙 / 1991 / 醫宗金鑑 / 대성문화사 : 448 ~
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UCI(KEPA) : I410-ECN-0101-2014-519-000281179