지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
이용수
초록· 키워드
오류제보하기
Parkin is known to be a tumor suppressor protein. Previously, we determined that parkin expression restores susceptibility to TNF-α-induced death of HeLa cells, a human cervical cancer cell line resistant to TNF-α-induced cell death. MMP-3 is a zinc-dependent protease recently reported to activate intracellular apoptotic signaling. In this study we examined the regulation of MMP-3 expression by parkin in TNF-α-treated HeLa cells. Furthermore, we investigated the signaling pathway involved in parkin-induced expression of MMP-3. We found that HeLa cells exhibit low levels of MMP-3 but is induced after introduction of the parkin gene into HeLa cells. Furthermore, MMP-3 expression increased further when parkin expressing cells were treated with TNF-α. Using chemical inhibitors of cell signaling pathways, we found that MEK-1 (PD98059), PI3K (LY294002), p38 MAPK (SB203580), and JNK inhibitors alleviated parkin-induced up-regulation of MMP-3. Finally, we show that TNF-α-induced cell death in parkin expressing cells is inhibited by using a MMP-3 inhibitor. These results suggest that parkin expression induces prolonged expression of MMP-3 via MEK-1, PI3K, MAPK, and JNK pathway in HeLa cells allowing the HeLa cells to become sensitive to TNF-α-induced cell death. These results implicate a role of MMP-3 in parkin-induced cell death in TNF-α treated HeLa cells.
목차
INTRODUCTION
MATERIALS AND METHODS
RESULTS
DISCUSSION
REFERENCES
참고문헌 (27)
참고문헌 신청
1. [학술지(정기간행물)] - Agirre X / 2006 / Abnormal methylation of the common PARK2 and PACRG promoter is associated with downregulation of gene expression in acute lymphoblastic leukemia and chronic myeloid leukemia / Int J Cancer 118 : 1945 ~ 1953
2. [학술지(정기간행물)] - Balkwill F / 2009 / Tumour necrosis factor and cancer / Nat Rev Cancer 9 : 361 ~ 371
3. [학술지(정기간행물)] - Borghaei RC / 2009 / NF-kappaB and ZBP-89 regulate MMP-3 expression via a polymorphic site in the promoter / Biochem Biophys Res Commun 382 : 269 ~ 273
4. [학술지(정기간행물)] - Choi DH / 2008 / A novel intracellular role of matrix metalloproteinase-3 during apoptosis of dopaminergic cells / JOURNAL OF NEUROCHEMISTRY 106 : 405 ~ 415
5. [학술지(정기간행물)] - Denison SR / 2003 / Characterization of FRA6E and its potential role in autosomal recessive juvenile parkinsonism and ovarian cancer / Genes Chromosomes Cancer 38 : 40 ~ 52
2. [학술지(정기간행물)] - Balkwill F / 2009 / Tumour necrosis factor and cancer / Nat Rev Cancer 9 : 361 ~ 371
3. [학술지(정기간행물)] - Borghaei RC / 2009 / NF-kappaB and ZBP-89 regulate MMP-3 expression via a polymorphic site in the promoter / Biochem Biophys Res Commun 382 : 269 ~ 273
4. [학술지(정기간행물)] - Choi DH / 2008 / A novel intracellular role of matrix metalloproteinase-3 during apoptosis of dopaminergic cells / JOURNAL OF NEUROCHEMISTRY 106 : 405 ~ 415
5. [학술지(정기간행물)] - Denison SR / 2003 / Characterization of FRA6E and its potential role in autosomal recessive juvenile parkinsonism and ovarian cancer / Genes Chromosomes Cancer 38 : 40 ~ 52
이 논문의 저자 정보
이 논문과 함께 이용한 논문
Septicemia Caused by Leuconostoc lactis with Intrinsic Resistance to Vancomycin in a Patient with Biliary Stent
Kyeong Seob Shin
,
Kyudong Han
,
Seung Bok Hong
대한의생명과학회지
2013
.09
Comparison of Molecular Characteristics of Extended Spectrum β-lactamase Producing Escherichia coli Strains Isolated from Patients with Urinary Tract Infections between 2 Time Periods of 1989 and 2010 at Gangwon Province in Korea
Min Pa
,
k
,
Soon Deok Pa
외 10명
대한의생명과학회지
2013
.09
Isoliquiritigenin Inhibits Ovalbumin-induced Cyclooxygenase-2 and Inducible Nitric Oxide Synthase Expression
Se Jin Lim
,
Hyung-Sun Youn
대한의생명과학회지
2013
.09
Direct Interaction Between Akt1 and Gcn5 and its Plausible Function on Hox Gene Expression in Mouse Embryonic Fibroblast Cells
Ji Hoon Oh
,
You
,
a Lee
외 2명
대한의생명과학회지
2013
.09
Endothelial Cells Isolated from the Bovine Corpus Luteum Synthesize Prostaglandin F2α Receptor
Sun‐Yeong Gwon
,
Ki‐Jong Rhee
,
Seunghyung Lee
대한의생명과학회지
2013
.09
최근 본 자료
댓글(0)
0
UCI(KEPA) : I410-ECN-0101-2014-520-003115706