지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
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Pluripotent stem cells (PSCs) have lots of potential in biomedical sciences owing to its potential to differentiate into any kind of cells in the body. However, it is still a challenge to culture PSCs on a large scale for application to regenerative medicine. Herein, we introduce a synthetic polymer that enables large-scale suspension culture of human PSCs. By employing suspension culture, it became unnecessary to use conventional substrata such as mouse embryonic fibroblast (MEF) or Matrigel<SUP>TM</SUP>, which are believed to be main causative sources of xenogeneic contamination in cultured human PSCs in vitro. Human PSCs were cultured in the medium in which elastin-like polypeptide (ELP) dissolved. The ELP in the medium became harden as temperature increases by transforming the medium into a semi-solid gel that supported growth of human PSCs in suspension. Gel-sol transition temperature of ELP can be adjusted by modifying the peptide sequence in which 5 amino acids, Val-Pro-Gly-Xaa-Gly, repeated sequentially. We constructed 3D suspension media having transition temperature around 33~35℃ using an ELP consisted of 40, 60, or 80 repeats of a monomer, which was Val-Pro-Gly-Val-Gly. Among the ELPs, ELP80 was chosen as the best ELP to support growth of human PSCs in suspension culture. This result suggests that the ELP80 can be a medium component for culturing human PSCs in large-scale.
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REFERENCES
참고문헌 (21)
참고문헌 신청
Amit M / 2011 / Dynamic suspension culture for scalable expansion of undifferentiated human pluripotent stem cells / Nat Protoc 6:572~579
Banki MR / 2005 / Simple bioseparations using self-cleaving elastin-like polypeptide tags / Nature Methods 2:659~661
Chilkoti A / 2006 / Stimulus responsive elastin biopolymers: Applications in medicine and biotechnology / Curr Opin Chem Biol 10:652~657
Di Zio K / 2003 / Mechanical properties of artificial protein matrices engineered for control of cell and tissue behavior / Macromolecules 36:1553~1558
Gerecht S / 2007 / Hyaluronic acid hydrogel for controlled self-renewal and differentiation of human embryonic stem cells / Proc Natl Acad Sci USA 104:11298~11303
Banki MR / 2005 / Simple bioseparations using self-cleaving elastin-like polypeptide tags / Nature Methods 2:659~661
Chilkoti A / 2006 / Stimulus responsive elastin biopolymers: Applications in medicine and biotechnology / Curr Opin Chem Biol 10:652~657
Di Zio K / 2003 / Mechanical properties of artificial protein matrices engineered for control of cell and tissue behavior / Macromolecules 36:1553~1558
Gerecht S / 2007 / Hyaluronic acid hydrogel for controlled self-renewal and differentiation of human embryonic stem cells / Proc Natl Acad Sci USA 104:11298~11303
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UCI(KEPA) : I410-ECN-0101-2014-500-003115753