The impact of exercise on axon regeneration processes has not been confirmed and the mechanisms underlying the benefit of exercise have not been determined in median nerve injured mouse model. The purpose of this study was to determined the effects of treadmill exercise on nerve regeneration in median nerve cut and sutured peripheral nerve regeneration mouse model in morphological and functional study methods. The exercise results were compared with that of the control and sedentary no exercised animals. The results of this study were summarized as follows. (1) Treadmill exercise increased the number of axon in the median nerve after PNI(Peripheral Nerve Injury) (2) Treadmill exercise induce the proper g-ratio and myelin thickness compare with sedentary no exercised group. This means exercise inhibits morphological recovery in the median nerv after PNI. (3) Treadmill exercise induces recovery of EMG results(CAMP and time delay(latency)) in the median nerve rather than sedentary no exercised group. (4) Treadmill exercise induces recovery of functional behavior (grip strength and wire grid holding) test results rather than sedentary no exercised group. These result means treadmill exercise profitable for induce the neuronal regeneration in the injured median nerve. The results of this study suggest that treadmill exercise induce the nerve regeneration in the injured median nerve and this effects were may offer a potential therapeutic adjunct to current nerve regeneration therapies in the future.