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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2014.7
- 수록면
- 1,025 - 1,035 (11page)
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This study assessed the effect of immobilized lipase on weak base styrene resin using polyethyleneimine (PEI) with cross-linking. Two procedures were used in this study. The first one, “mono-layer” lipase immobilization, involves washing PEI after adsorption. The second procedure, “multi-layer” lipase immobilization, has no washing before the cross-linking step. Treverlite XS-100200 (weak base styrene resin) was immersed with PEI solution (2.2 mg/mL). Lipase AH (from Burkholderia cepacia) was adsorbed onto the support coated with PEI before cross-linking with glutaraldehyde. Structured lipid was synthesized by immobilized lipase-catalyzed interesterification using canola oil, palmitic ethyl ester (PEE), and stearic ethyl ester (StEE). Total fatty acid contents of triacylglycerol (TAG) in structured lipids were analyzed to investigate activity, properties, and reusability of immobilized lipases. Activities of immobilized lipases on the multi-layer and mono-layer increased at a high concentration (8 mg/mL) of lipase solution used for immobilization. The results show that immobilized lipase with the mono-layer method at pH 8.0 on resin had the highest total saturated fatty acid content (26.17 area%). Activity of immobilized lipase with the multi-layer method at pH 7.5 on support was lower than that of the mono-layer, but total saturated fatty acid content was 16.79 area% higher than that of lipase AH (15.01 area%).
목차
ABSTRACT
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재료 및 방법
결과 및 고찰
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REFERENCES
참고문헌 (28)
참고문헌 신청
1. [학술지(정기간행물)] - Kim JK / 2004 / Synthesis and characterization of nanoporous silica support for enzyme immobilization / Colloids Surf, A 241 : 113 ~ 117
2. [학술지(정기간행물)] - Lee KT / 1998 / Immobilization of lipases on clay, Celite 545, diethylaminoethyl-, and carboxymethyl-Sephadex and their interesterification activity / Biotechnol Tech 12 : 381 ~ 384
3. [학술지(정기간행물)] - Malcata FX / 1992 / Kinetics and mechanism of reactions catalyzed by immobilized lipases / Enzyme Microb Technol 12 : 426 ~ 446
4. [학술지(정기간행물)] - Balco VM / 1996 / Bioreactors with immobilized lipases : state of the art / Enzyme Microb Technol 18 : 392 ~ 416
5. [학술지(정기간행물)] - Ye P / 2007 / Adsorption and activity of lipase from candida rugosa on the chitosan-modified poly (acrylonitrile-co-maleic acid) membrane surface / Colloids Surf, B 60 : 62 ~ 67
2. [학술지(정기간행물)] - Lee KT / 1998 / Immobilization of lipases on clay, Celite 545, diethylaminoethyl-, and carboxymethyl-Sephadex and their interesterification activity / Biotechnol Tech 12 : 381 ~ 384
3. [학술지(정기간행물)] - Malcata FX / 1992 / Kinetics and mechanism of reactions catalyzed by immobilized lipases / Enzyme Microb Technol 12 : 426 ~ 446
4. [학술지(정기간행물)] - Balco VM / 1996 / Bioreactors with immobilized lipases : state of the art / Enzyme Microb Technol 18 : 392 ~ 416
5. [학술지(정기간행물)] - Ye P / 2007 / Adsorption and activity of lipase from candida rugosa on the chitosan-modified poly (acrylonitrile-co-maleic acid) membrane surface / Colloids Surf, B 60 : 62 ~ 67
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