지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2014.6
- 수록면
- 131 - 138 (8page)
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초록· 키워드
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In this study, we isolated two novel chitinase producing bacterial strains from yellow loess samples collected from Jullanamdo province. The chitinase producing bacteria were isolated based on the zone size of clearance in the chitin agar plates. Both of them were gram positive, rod (2~3×0.3~0.4 μm), spore-forming, and motility positive. They were facultative anaerobic, catalase positive and hydrolyzed starch, gelatin, and casein. From the 16s rRNA gene sequence analysis, the isolates were labeled as Bacillus licheniformis KYLSCU01 and B. licheniformis KYLS-CU02. The isolates showed higher extracellular chitinase activities than B. licheniformis ATCC 14580 as a control. The optimum temperature and pH for chitinase production were 40oC and pH 7.0, respectively. Response Surface Methodology (RSM) was used to optimize the culture medium for efficient production of the chitinase. Under this optimal condition, 1.5 times higher chitinase activity of B. licheniformis KYLS-CU02 was obtained. Extracellular chitinases of the two isolates were purified through ammonium sulfate precipitation and anion-exchange DEAE-cellulose column chromatography. The specific activities of purified chitinase from B. licheniformis KYLS-CU01 and B. licheniformis KYLS-CU02 were 7.65 and 5.21 U/mg protein, respectively. The molecular weights of the two purified chitinases were 59 kDa. Further, the purified chitinase of B. licheniformis KYLS-CU01 showed high antifungal activity against Fusarium sp.. In conclusion, these two bacterial isolates can be used as a biopesticide to control pathogenic fungi.
목차
Abstract
1. INTRODUCTION
2. MATERIALS AND METHOD
3. RESULTS AND DISCUSSION
4. CONCLUSION
REFERENCES
참고문헌 (18)
참고문헌 신청
1. [인터넷자원] - / / Statistics Korea Ministry of Agriculture Food and Rural Arrairs Consumer Policy Division. Use of chemical pesticides and fetilizers
2. [학술지(정기간행물)] - Nam, H. S. / 2011 / Environmentally-friendly agriculture and biotic pesticide / Korean Industrial Chemistry News 14 : 12 ~ 18
3. [학술지(정기간행물)] - Mahadevan, B. / 1997 / Properties of the chitinase of the antifungal biocontrol agent Streptomyces lydicus WYEC 108 / Enzyme Microb. Technol 20 : 489 ~ 493
4. [학술지(정기간행물)] - Raaijmakers, J. M. / 1995 / Doseresponse relationships in biological control of Fusarium wilt of radish by Pseudomonas spp / Phytopathol 85 : 1075 ~ 1081
5. [학술지(정기간행물)] - Boller, T. / 1983 / Chitinase in bean leaves: induction by ethylene, purification, properties, and possible function / Planta 157 : 22 ~ 31
2. [학술지(정기간행물)] - Nam, H. S. / 2011 / Environmentally-friendly agriculture and biotic pesticide / Korean Industrial Chemistry News 14 : 12 ~ 18
3. [학술지(정기간행물)] - Mahadevan, B. / 1997 / Properties of the chitinase of the antifungal biocontrol agent Streptomyces lydicus WYEC 108 / Enzyme Microb. Technol 20 : 489 ~ 493
4. [학술지(정기간행물)] - Raaijmakers, J. M. / 1995 / Doseresponse relationships in biological control of Fusarium wilt of radish by Pseudomonas spp / Phytopathol 85 : 1075 ~ 1081
5. [학술지(정기간행물)] - Boller, T. / 1983 / Chitinase in bean leaves: induction by ethylene, purification, properties, and possible function / Planta 157 : 22 ~ 31
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