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자료유형
학술저널
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대한바이러스학회 JOURNAL OF BACTERIOLOGY AND VIROLOGY 大韓바이러스學會誌 제12권 제1호
발행연도
1982.6
수록면
35 - 41 (7page)

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Recently it was known that rotavirus is one of major causing agents in childhood diarrheas throughout the world. A study on viral aetiological agents of childhood diarrhea was performed with no previous report in Korea. Fiftytwo stool specimens collected from diarrhea patients in Hanyang Medical Centre during the epidemic season of late autumn to early winter were observed by transmission electron microscopy (TEM). Fifty to 70 nm wheel-like rotavirus particles were present in purified material by centrifugation in the forms of double or single shelled, or empty capsids. Additionally adenovirus-like particles and bacteriophages could be observed in the same specimens with their significance as a causing agent unknown. Enzyme immuno assay (EIA) was also performed on the same specimens for rotavirus detection by immunological method, EIA results were coincided with those of electron microscopy in virus detection except 5 cases which were positive in EIA and negative in EM. Comparisions of EM and EIA methods showed that EIA has advantages over EM in sensitivity, cost, easiness and running time. On the other hand EM has advantage in its ability observing morphological characteristics of virus particles present in stool. EIA results on the above 52 specimens showed that 75% of patients clinically diagnosed as acute gastroenteritis and 61.5% of patients with diarrhea symptoms during rotavirus epidemic season were caused by rotavirus.

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