지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2008.12
- 수록면
- 137 - 142 (6page)
이용수
초록· 키워드
오류제보하기
CD11c, also known as integrin alpha x, is one of the optimum markers of dendritic cells. However, the regulation of the CD11c expression in mouse has not been identified yet. In this study, in order to analyze the regulation of CD11c expression, the promoter of CD11c was cloned and characterized. Methods: To identify the promoter portion, various sizes of what are considered to be CD11c promoter fragments was amplified by polymerase chain reaction (PCR), using mouse genomic DNA as a template. After sequence was obtained, these fragments were transfected into various cell lines including mouse dendritic cell lines such as JAWSII and DC2.4 and L929 as control cell line.. The promoter activity of three promoter fragments was measured and compared by luciferase activity in the transfected cells. Results: Three clones with size of 1kb, 3kb and 6kb were obtained from mouse genomic DNA. Flow cytometry analysis of JAWSII cells revealed that 52% of the cells expressed CD11c, which was confirmed by RT-PCR analysis. On the contrary, L929 and DC 2.4 cells did not express CD11c. The CD11c+ JAWSII cells were enriched from 52% to 90% with cell sorter. The comparative luciferase activity analyisis demonstrated that the region responsible for tissue specific expression was contained within -3 kb and the clone with size of 3 kb particularly showed higher luciferase activity than 6 kb and 1 kb clones. Conclusion: The CD11c promoter region containing the region responsible for tissue specificity was successfully cloned and -3 kb region showed the highest activity.
목차
등록된 정보가 없습니다.
참고문헌 (26)
참고문헌 신청
1. [학술지(정기간행물)] - Lanier LL / 1985 / p150/95, third member of the LFA-1/CR3 polypeptide family identified by anti-Leu M5 monoclonal antibody / Eur J Immunol 15 : 713 ~ 718
2. [학술지(정기간행물)] - Springer TA / 1986 / p150,95, the third member of the Mac-1, LFA-1 human leukocyte adhesion glycoprotein family / J Immunol 136 : 240 ~ 245
3. [학술지(정기간행물)] - Sanchez-Madrid F / 1983 / A human leukocyte differentiation antigen family with distinct alpha-subunits and a common beta-subunit: the lymphocyte function-associated antigen (LFA-1), the C3bi complement receptor (OKM1/Mac-1), and the p150,95 molecule / J Exp Med 158 : 1785 ~ 1803
4. [학술지(정기간행물)] - Corbi AL / 1987 / cDNA cloning and complete primary structure of the alpha subunit of a leukocyte adhesion glycoprotein, p150,95 / EMBO J 6 : 4023 ~ 4028
5. [학술지(정기간행물)] - Malhotra V / 1986 / Ligand binding by the p150,95 antigen of U937 monocytic cells: properties in common with complement receptor type 3 (CR3) / Eur J Immunol 16 : 1117 ~ 1123
2. [학술지(정기간행물)] - Springer TA / 1986 / p150,95, the third member of the Mac-1, LFA-1 human leukocyte adhesion glycoprotein family / J Immunol 136 : 240 ~ 245
3. [학술지(정기간행물)] - Sanchez-Madrid F / 1983 / A human leukocyte differentiation antigen family with distinct alpha-subunits and a common beta-subunit: the lymphocyte function-associated antigen (LFA-1), the C3bi complement receptor (OKM1/Mac-1), and the p150,95 molecule / J Exp Med 158 : 1785 ~ 1803
4. [학술지(정기간행물)] - Corbi AL / 1987 / cDNA cloning and complete primary structure of the alpha subunit of a leukocyte adhesion glycoprotein, p150,95 / EMBO J 6 : 4023 ~ 4028
5. [학술지(정기간행물)] - Malhotra V / 1986 / Ligand binding by the p150,95 antigen of U937 monocytic cells: properties in common with complement receptor type 3 (CR3) / Eur J Immunol 16 : 1117 ~ 1123
함께 읽어보면 좋을 논문
논문 유사도에 따라 DBpia 가 추천하는 논문입니다. 함께 보면 좋을 연관 논문을 확인해보세요!
-
Role for CD40 and CD40L Expression in Generating CD8 T Cell Response to Minor Histcompatibility Antigen, H60
Immune Network
2007 .12
-
CD40L 발현 K562 세포주를 이용한 시험관내 B 세포 증식과 항원제공능 증가
Immune Network
2007 .06
-
Expression and Function of TLR2 on CD4 Versus CD8 T Cells
Immune Network
2009 .08
-
Regulation of Inflammation by Bidirectional Signaling through CD137 and Its Ligand
Immune Network
2012 .10
-
CD43 Expression Regulated by IL-12 Signaling Is Associated with Survival of CD8 T Cells
Immune Network
2010 .10
-
Extrinsic Acquisition of CD80 by Antigen-Specific CD8⁺ T Cells Regulates Their Recall Immune Responses to Acute Viral Infection
Immune Network
2019 .08
-
Cross-linking of CD80 and CD86 Diminishes Expression of CD54 on EBV-transformed B Cells through Inactivation of RhoA and Ras
Immune Network
2011 .12
-
CD137-CD137 Ligand Interactions in Inflammation
Immune Network
2009 .06
-
Requirement of CD4 Help for Induction of CD8 T Cell Response Specific for Virally Derived H60
Immune Network
2012 .06
-
Two Distinct Subsets Are Identified from the Peritoneal Myeloid Mononuclear Cells Expressing both CD11c and CD115
Immune Network
2019 .06
-
Adhesion of CD40-stimulated Germinal Center B Cells to HK Cells Employs the CD11a/CD18-CD54 Interactions
Immune Network
2003 .09
-
결장암에 대한 활성 자연살해세포의 항암효능
약학회지
2010 .01
-
마행감석탕가감방(麻杏甘石湯加減方)이 천식모델 생쥐의 CD3, CD4, CD8 세포에 미치는 영향
논문집 : 대전대학교 한의학연구소. 한의학편
2008 .01
-
비소세포 폐암종의 CD44s 와 Cycylooxygenase-2 발현
Journal of Pathology and Translational Medicine
2006 .01
-
간세포암에서 CD40 과 CD154의 발현
Annals of Hepato-Biliary-Pancreatic Surgery
2007 .12
-
T-Cells Modified with CD70 as an Alternative Cellular Vaccine for Antitumor Immunity
Cancer Research and Treatment
2020 .01
-
비소세포폐암종에서 CD44s, CD44v5 및 CD44v6 발현의 임상병리학적 의의
Journal of Pathology and Translational Medicine
2004 .01
-
Significance of Expression of CD44s and CD44v6 in Colorectal Cancer
대한의생명과학회지
2002 .09
이 논문의 저자 정보
이 논문과 함께 이용한 논문
최근 본 자료
댓글(0)
0