지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 대한외과학회 Annals of Surgical Treatment and Research Annals of Surgical Treatment and Research Vol.90 No.4
- 발행연도
- 2016.4
- 수록면
- 183 - 193 (11page)
이용수
초록· 키워드
오류제보하기
Purpose: To determine CD133<SUP>+</SUP> cells defined as cancer stem cells (CSCs) in colon cancer, we examined whether CD133<SUP>+</SUP> clones in HCT116 demonstrate known features of CSCs like sphere-forming ability, chemodrug-resistance, and metastatic potential.
Methods: Magnetic cell isolation and cell separation demonstrated that <1% of HCT116 cells expressed CD133, with the remaining cells being CD133<SUP>-</SUP> clones. In colon cancer cells, radioresistance is also considered a CSC characteristic. We performed clonogenic assay using 0–4 Gy g-irradiation.
Results: Interestingly, there were no differences between HCT116 parental and HCT116 CD133<SUP>+</SUP> clones when the cells comprised 0.5% of the total cells, and CD133<SUP>-</SUP> clone demonstrated radiosensitive changes compared with parental and CD133<SUP>+</SUP> clones. Comparing gene expression profiles between sphere-forming and nonforming culture conditions of HCT116 subclones by whole RNA sequencing failed to obtain specific genes expressed in CD133<SUP>+</SUP> clones.
Conclusion: Despite no differences of gene expression profiles in monolayer attached culture conditions of each clone, sphere-forming conditions of whole HCT116 subclones, parental, CD133<SUP>+</SUP>, and CD133<SUP>-</SUP> increased 1,761 coding genes and downregulated 1,384 genes related to CSCs self-renewal and survival. Thus, spheroid cultures of HCT116 cells could be useful to expand colorectal CSCs rather than clonal expansion depending on CD133 expressions.
Methods: Magnetic cell isolation and cell separation demonstrated that <1% of HCT116 cells expressed CD133, with the remaining cells being CD133<SUP>-</SUP> clones. In colon cancer cells, radioresistance is also considered a CSC characteristic. We performed clonogenic assay using 0–4 Gy g-irradiation.
Results: Interestingly, there were no differences between HCT116 parental and HCT116 CD133<SUP>+</SUP> clones when the cells comprised 0.5% of the total cells, and CD133<SUP>-</SUP> clone demonstrated radiosensitive changes compared with parental and CD133<SUP>+</SUP> clones. Comparing gene expression profiles between sphere-forming and nonforming culture conditions of HCT116 subclones by whole RNA sequencing failed to obtain specific genes expressed in CD133<SUP>+</SUP> clones.
Conclusion: Despite no differences of gene expression profiles in monolayer attached culture conditions of each clone, sphere-forming conditions of whole HCT116 subclones, parental, CD133<SUP>+</SUP>, and CD133<SUP>-</SUP> increased 1,761 coding genes and downregulated 1,384 genes related to CSCs self-renewal and survival. Thus, spheroid cultures of HCT116 cells could be useful to expand colorectal CSCs rather than clonal expansion depending on CD133 expressions.
목차
INTRODUCTION
METHODS
RESULTS
DISCUSSION
REFERENCES
참고문헌 (24)
참고문헌 신청
Kreso A, Dick JE. Evolution of the cancer stem cell model. Cell Stem Cell 2014;14: 275-91.
Zeuner A, Todaro M, Stassi G, De Maria R. Colorectal cancer stem cells: from the crypt to the clinic. Cell Stem Cell 2014;15: 692-705.
O'Brien CA, Pollett A, Gallinger S, Dick JE. A human colon cancer cell capable of initiating tumour growth in immunodeficient mice. Nature 2007;445:106-10.
Ricci-Vitiani L, Lombardi DG, Pilozzi E, Biffoni M, Todaro M, Peschle C, et al. Identification and expansion of human colon-cancer-initiating cells. Nature 2007; 445:111-5.
Todaro M, Alea MP, Di Stefano AB, Cammareri P, Vermeulen L, Iovino F, et al. Colon cancer stem cells dictate tumor growth and resist cell death by production of interleukin-4. Cell Stem Cell 2007; 1:389-402.
Zeuner A, Todaro M, Stassi G, De Maria R. Colorectal cancer stem cells: from the crypt to the clinic. Cell Stem Cell 2014;15: 692-705.
O'Brien CA, Pollett A, Gallinger S, Dick JE. A human colon cancer cell capable of initiating tumour growth in immunodeficient mice. Nature 2007;445:106-10.
Ricci-Vitiani L, Lombardi DG, Pilozzi E, Biffoni M, Todaro M, Peschle C, et al. Identification and expansion of human colon-cancer-initiating cells. Nature 2007; 445:111-5.
Todaro M, Alea MP, Di Stefano AB, Cammareri P, Vermeulen L, Iovino F, et al. Colon cancer stem cells dictate tumor growth and resist cell death by production of interleukin-4. Cell Stem Cell 2007; 1:389-402.
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UCI(KEPA) : I410-ECN-0101-2016-514-002860559