지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2016.12
- 수록면
- 201 - 212 (12page)
이용수
초록· 키워드
오류제보하기
Species identification is an important item to characterize unidentified bacterial pathogens in developing and managing bacterial resources. In this study, unidentified pathogens based on the results of an automated identification system were identified using matrix assisted laser desorption ionization-time of flight mass spectrometry (MALD-TOF MS) and 16S rRNA gene analysis for development of national resources in the National Culture Collection for Pathogens (NCCP) in Korea. A total of 437 unidentified strains from branch banks of the NCCP were collected, and 16S rRNA and dnaJ gene sequencing, as well as MALDI-TOF MS analysis were performed to identify bacterial species. The mass spectra extracted were analyzed. Twelve strains exhibiting less than 98.65% similarity in 16S rRNA gene were selected as the primary candidates for novel species, and 21 strains exhibiting 98.65~99.0% similarity in 16S rRNA gene were selected as possible candidates for novel species. Among them, strain 32, belonging to Dermabacter sp., was finally selected as a possible strain representing a novel species and 14 unidentified bacterial strains using automated phenotypic identification system were newly registered at NCCP. The present study showed that unidentified pathogens using the automated phenotypic identification system were efficiently identified using the combination of MALDI-TOF MS and 16S rRNA gene analysis, and developed to the national resources in NCCP.
목차
INTRODUCTION
MATERIALS AND METHODS
RESULTS
DISCUSSION
REFERENCES
참고문헌 (27)
참고문헌 신청
[학술지(정기간행물)] - Yu WS / 2015 / Resource development of unidentified Human Pathogens using automated identification systems / Public Health Weekly Report 8 : 990 ~ 997
[학술지(정기간행물)] - Funke G / 1998 / Evaluation of the VITEK 2 system for rapid identification of medically relevant gram-negative rods / J Clin Microbiol 36 : 1948 ~ 1952
[학술지(정기간행물)] - Benagli C / 2012 / A rapid MALDI-TOF MS identification database at genospecies level for clinical and environmental Aeromonas strains / PLoS One 7 : e48441 ~
[학술지(정기간행물)] - Christner M / 2014 / Rapid MALDI-TOF mass spectrometry strain typing during a large outbreak of Shiga-Toxigenic Escherichia coli / PLoS One 9 : e101924 ~
[학술지(정기간행물)] - Josten M / 2013 / Analysis of the matrix-assisted laser desorption ionization-time of flight mass spectrum of Staphylococcus aureus identifies mutations that allow differentiation of the main clonal lineages / J Clin Microbiol 51 : 1809 ~ 1817
[학술지(정기간행물)] - Funke G / 1998 / Evaluation of the VITEK 2 system for rapid identification of medically relevant gram-negative rods / J Clin Microbiol 36 : 1948 ~ 1952
[학술지(정기간행물)] - Benagli C / 2012 / A rapid MALDI-TOF MS identification database at genospecies level for clinical and environmental Aeromonas strains / PLoS One 7 : e48441 ~
[학술지(정기간행물)] - Christner M / 2014 / Rapid MALDI-TOF mass spectrometry strain typing during a large outbreak of Shiga-Toxigenic Escherichia coli / PLoS One 9 : e101924 ~
[학술지(정기간행물)] - Josten M / 2013 / Analysis of the matrix-assisted laser desorption ionization-time of flight mass spectrum of Staphylococcus aureus identifies mutations that allow differentiation of the main clonal lineages / J Clin Microbiol 51 : 1809 ~ 1817
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UCI(KEPA) : I410-ECN-0101-2017-475-001976861