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논문 기본 정보

자료유형
학술저널
저자정보
Jinsoo Chung (Chungnam National University) Jae-yeong Han (Chungnam National University) Jungkyu Kim (Chungnam National University) Hyekyoung Ju (Chungnam National University) Junsu Gong (Chungnam National University) Eun-young Seo (Chungnam National University) Su Ryun Choi (Chungnam National University) Yong Pyo Lim (Chungnam National University) John Hammond (Floral and Nursery Plants Research Unit) Hyoun-Sub Lim (Chungnam National University)
저널정보
충남대학교 농업과학연구소 Korean Journal of Agricultural Science Korean Journal of Agricultural Science Vol.43 No.4
발행연도
2016.12
수록면
567 - 574 (8page)

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This survey was conducted in 2015, following up on theed tthe occurrence of Turnip mosaic virus (TuMV) nationwide in radish and Chinese cabbage fields of 28 cities in South Korea. A total of 152 samples of Raphanus sativus and 29 samples of Brassica rapa, showing virus-like symptoms, were collected. Among these, 107 B. rapa samples and 9 B. rapa samples were positive for TuMV when analyzed by RT-PCR. The TuMV strains found in the two crops showed 99% homology in nucleotide and amino acid sequences of coat protein to each other. Furthermore, their sequences showed 99% homology to the sequences of TuMV isolates R007 (GenBank: KU140420) and R041 (GenBank: KU140421) that were collected in 2014. These results suggested TuMV isolated from radish and cabbage in 2015 were the same strain as the isolates R007 and R041 collected in 2014. A screening test was conducted using these two isolates to select TuMV-resistant B. rapa lines out of 167 B. rapa breeding lines.and identified eight lines resistant to R007 (Kenshin, 279002, 279012, 279064, 279081, MP, C-21, HKC-004) and nine lines resistant to R041 (C-26, HKC-005, 11Su-4, 11Su-5, 11Su-7, 11Su-8, Tian Jin Lv Qing Ma Ye, CNU_141193, Jing Lv 60). Our prior data indicated 4.24% difference in sequences between the two isolates and these can serve as potential tools to develop B. rapa markers to screen for resistance against TuMV strainsin breeding populations.

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UCI(KEPA) : I410-ECN-0101-2017-520-002299114