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논문 기본 정보
- 자료유형
- 학술대회자료
- 저자정보
- 발행연도
- 2021.10
- 수록면
- 210 - 210 (1page)
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초록· 키워드
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Many of the recessive virus-resistance genes in plants encode eukaryotic translation initiation factors (eIFs), including eIF4E, eIF4G, and related proteins. Particularly, eIF4E and its isoform eIF(iso)4E are crucial for viral infection and act as recessive resistance genes against various potyviruses in a broad range of plants. In the present study, we used Clustered Regularly Interspaced Palindromic Repeats/CRISPR-associated protein 9 (CRISPR/ Cas9)-mediated directed mutagenesis to test whether novel sequence-specific mutations at eIF4E1 in Brassica rapa (Chinese cabbage) cv. ‘Seoul’ could confer enhanced resistance to potyviruses. This approach produced mutations in the transgene-free E1 generation. Sequence analysis of eIF4E1 from E0 transgenic plants expressing Cas9 and eIF4E-sgRNA transcripts identifi ... 전체 초록 보기
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