Isolation and Genotyping of Toxoplasma gondii in Brazilian Dogs

Jamille Rodrigues da Silva; Bianca Mendes Maciel; Luana Karla Nogueira de Santana Souza Santos; Fábio Santos Carvalho; Daniele de Santana Rocha; Carlos Wilson Gomes Lopes; George Rêgo Albuquerque

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자료유형: 학술저널

저자정보: Jamille Rodrigues da Silva (UESC) Bianca Mendes Maciel (UESC) Luana Karla Nogueira de Santana Souza Santos (UESC) Fábio Santos Carvalho (UESC) Daniele de Santana Rocha (UESC) Carlos Wilson Gomes Lopes (UFRRJ) George Rêgo Albuquerque (UESC)

저널정보: 대한기생충학열대의학회 Parasites, Hosts and Diseases The Korean Journal of Parasitology Vol.55 No.3

발행연도: 2017.6

수록면: 239 - 246 (8page)

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Strains of Toxoplasma gondii in Brazil are highly genetically diverse compared to strains from North America and Europe. Dogs are epidemiologically important because they act as sentinels for T. gondii infections in humans and are good indicators of environmental contamination. The aim of this study was to isolate and genetically characterize T. gondii strains from tissues of naturally infected Brazilian dogs. For this study, 21 blood samples were collected from dogs at the Zoonosis Control Centers of Ilhéus and Itabuna cities, Bahia, Brazil. The sera were examined for T. gondii antibodies using the indirect hemagglutination test. Brains and hearts of seropositive dogs were bioassayed in mice to isolate and characterize T. gondii parasites by PCR-RFLP using 10 genetic markers (SAG1, newSAG2, SAG3, BTUB, c22-8, c29-2, GRA6, PK1, APICO, and L358). However, T. gondii was isolated from only 4 (57.1%) dogs, designated TgDgBr6, 13, 17, and 21. All strains were virulent, causing clinical changes (rough hair coat, lethargy, and abdominal distention) and the death of all mice within 8-20 days after inoculation. Genetic analysis of these 4 T. gondii isolates revealed 4 distinct genotypes with different clonal lineage combinations (types I, II, and III) and 2 atypical alleles. Using PCR-RFLP with several markers, this study contributes to evaluations of the genetic diversity of strains circulating in Brazil.

#Toxoplasma gondii #dog #genotype #toxoplasmosis #Brazil

참고문헌 (30)

참고문헌 신청

[단행본] - Dubey JP / 1988 / Toxoplasmosis of Animals and Man / CRC : 1 ~ 220

[학술지(정기간행물)] - Dubey JP / 2003 / Toxoplasma gondii, Neospora caninum, Sarcocystis neurona, and Sarcocystis canis-like infections in marine mammals / Vet Parasitol 116 : 275 ~ 296

[학술지(정기간행물)] - Ribeiro LA / 2015 / Detection of Toxoplasma gondii DNA in Brazilian oysters(Crassostrea rhizophorae) / Genet Mol Res 14 : 4658 ~ 4665

[학술지(정기간행물)] - Dubey JP / 2012 / Toxoplasmosis in humans and animals in Brazil: high prevalence, high burden of disease and epidemiology / Parasitology 139 : 1375 ~ 1424

[학술지(정기간행물)] - Dubey JP / 2004 / Toxoplasmosis-a waterborne zoonosis / Vet Parasitol 126 : 57 ~ 72

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