지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2017.10
- 수록면
- 602 - 607 (6page)
이용수
초록· 키워드
오류제보하기
Background: Panax ginseng is a traditional herb used for medicinal purposes in eastern Asia. P. ginseng contains various ginsenosides with pharmacological effects. In this study, floralginsenoside A (FGA), ginsenoside Rd (GRD), and ginsenoside Re (GRE) were purified from P. ginseng berry.
Methods: Chemical structures of FGA, GRD, and GRE were determined based on spectroscopic methods, including fast atom bombardment mass spectroscopy, ID-nuclear magnetic resonance, and infrared spectroscopy. Inhibitory activities of these compounds on melanogenesis were studied by measuring the expression of protein and melanin content in the melan-a cell line. This inhibitory activity was confirmed by observing pigmentation and tyrosinase activities of zebrafish.
Results: GRD, GRE, and FGA were not cytotoxic at concentrations less than 20mM, 80mM, and 160mM in melan-a cells, respectively. GRD, GRE, and FGA inhibited melanin biosynthesis in melan-a cells by 15.2%, 22.9%, and 23.9% at 20mM, 80mM, and 160mM, respectively. FGA was observed to display the most potent inhibitory effect. In addition, FGA decreased microphthalmia-associated transcription factor protein expression in a dose-dependent manner. Moreover, FGA induced extracellular signal-regulated kinase phosphorylation level in melan-a cells. In addition, melanin pigment content and tyrosinase activity in zebrafish treated with FGA at160mM were reduced.
Conclusion: FGA showed the most potent inhibition of melanogenesis in both in vitro and in vivo studies. This study suggests that FGA purified from P. ginseng may be an effective melanogenesis inhibitor.
Methods: Chemical structures of FGA, GRD, and GRE were determined based on spectroscopic methods, including fast atom bombardment mass spectroscopy, ID-nuclear magnetic resonance, and infrared spectroscopy. Inhibitory activities of these compounds on melanogenesis were studied by measuring the expression of protein and melanin content in the melan-a cell line. This inhibitory activity was confirmed by observing pigmentation and tyrosinase activities of zebrafish.
Results: GRD, GRE, and FGA were not cytotoxic at concentrations less than 20mM, 80mM, and 160mM in melan-a cells, respectively. GRD, GRE, and FGA inhibited melanin biosynthesis in melan-a cells by 15.2%, 22.9%, and 23.9% at 20mM, 80mM, and 160mM, respectively. FGA was observed to display the most potent inhibitory effect. In addition, FGA decreased microphthalmia-associated transcription factor protein expression in a dose-dependent manner. Moreover, FGA induced extracellular signal-regulated kinase phosphorylation level in melan-a cells. In addition, melanin pigment content and tyrosinase activity in zebrafish treated with FGA at160mM were reduced.
Conclusion: FGA showed the most potent inhibition of melanogenesis in both in vitro and in vivo studies. This study suggests that FGA purified from P. ginseng may be an effective melanogenesis inhibitor.
목차
ABSTRACT
1. Introduction
2. Materials and methods
3. Results and discussion
References
참고문헌 (38)
참고문헌 신청
[학술지(정기간행물)] - Kadekaro AL / 2005 / Alpha-melanocortin and endothelin-1 activate antiapoptotic pathways and reduce DNA damage in human melanocytes / Cancer Res 65 : 4292 ~ 4299
[학술지(정기간행물)] - Chiang HM / 2014 / Hydroalcoholic extract of Rhodiola rosea L. (Crassulaceae) and its hydrolysate inhibit melanogenesis in B16F0 cells by regulating the CREB/MITF/tyrosinase pathway / Food Chem Toxicol 65 : 129 ~ 139
[학술지(정기간행물)] - Hearing Jr VJ / 1987 / Mammalian monophenol monooxygenase (tyrosinase): purification, properties, and reactions catalyzed / Methods Enzymol 142 : 154 ~ 165
[학술지(정기간행물)] - Briganti S / 2003 / Chemical and instrumental approaches to treat hyperpigmentation / Pigment Cell Res 16 : 101 ~ 110
[학술지(정기간행물)] - Fujimoto N / 1998 / Induction of thyroid tumours in(C57BL/6N X C3H/N)F1 mice by oral administration of kojic acid / Food Chem Toxicol 36 : 697 ~ 703
[학술지(정기간행물)] - Chiang HM / 2014 / Hydroalcoholic extract of Rhodiola rosea L. (Crassulaceae) and its hydrolysate inhibit melanogenesis in B16F0 cells by regulating the CREB/MITF/tyrosinase pathway / Food Chem Toxicol 65 : 129 ~ 139
[학술지(정기간행물)] - Hearing Jr VJ / 1987 / Mammalian monophenol monooxygenase (tyrosinase): purification, properties, and reactions catalyzed / Methods Enzymol 142 : 154 ~ 165
[학술지(정기간행물)] - Briganti S / 2003 / Chemical and instrumental approaches to treat hyperpigmentation / Pigment Cell Res 16 : 101 ~ 110
[학술지(정기간행물)] - Fujimoto N / 1998 / Induction of thyroid tumours in(C57BL/6N X C3H/N)F1 mice by oral administration of kojic acid / Food Chem Toxicol 36 : 697 ~ 703
함께 읽어보면 좋을 논문
논문 유사도에 따라 DBpia 가 추천하는 논문입니다. 함께 보면 좋을 연관 논문을 확인해보세요!
-
Predictive analyses for balance and gait based on trunk performance using clinical scales in persons with stroke
Physical therapy rehabilitation science
2018 .01
-
뇌졸중 환자를 대상으로 실시한 한글판 기능적 보행평가의 타당도
한국전문물리치료학회지
2016 .01
-
Effect of ginseng and ginsenosides on melanogenesis and their mechanism of action
Journal of Ginseng Research
2015 .01
-
Effect of ginseng and ginsenosides on melanogenesis and their mechanism of action
Journal of Ginseng Research
2015 .07
-
Pharmacological and medical applications of Panax ginseng and ginsenosides : a review for use in cardiovascular diseases
Journal of Ginseng Research
2018 .06
-
Ultrasound-guided percutaneous nephrolithotomy: Advantages and limitations
Investigative and Clinical Urology
2017 .01
-
Effects of Panax ginseng and ginsenosides on oxidative stress and cardiovascular diseases: pharmacological and therapeutic roles
Journal of Ginseng Research
2022 .01
-
Effects of Korean ginseng berry on skin antipigmentation and antiaging via FoxO3a activation
Journal of Ginseng Research
2017 .07
-
Pharmacological potential of ginseng and its major component ginsenosides
Journal of Ginseng Research
2021 .03
-
Implications of red Panax ginseng in oxidative stress associated chronic diseases
Journal of Ginseng Research
2017 .04
-
Functional role of ginseng-derived compounds in cancer
Journal of Ginseng Research
2018 .06
-
Metabolomic understanding of intrinsic physiology in Panax ginseng during whole growing seasons
Journal of Ginseng Research
2019 .10
-
Cornus officinalis Methanol Extract Upregulates Melanogenesis in Melan-a Cells
Toxicological Research
2015 .06
-
Korean Red Ginseng extract ameliorates melanogenesis in humans and induces antiphotoaging effects in ultraviolet Beirradiated hairless mice
Journal of Ginseng Research
2020 .05
-
In situ analysis of chemical components induced by steaming between fresh ginseng, steamed ginseng, and red ginseng
Journal of Ginseng Research
2017 .07
-
고려인삼(Panax ginseng)과 미국삼(Panax quinquefolium)의 승열작용에 대한 비교 고찰
생약학회지(Korean Journal of Pharmacognosy)
2018 .09
-
Chemical diversity of ginseng saponins from Panax ginseng
Journal of Ginseng Research
2015 .10
-
Unraveling dynamic metabolomes underlying different maturation stages of berries harvested from Panax ginseng
Journal of Ginseng Research
2020 .05
-
Lipids in Ginseng (Panax ginseng) and Their Analysis
Natural Product Sciences
2018 .03
-
저령 추출물의 멜라닌 생성억제 작용
Korean Journal of Acupuncture
2020 .01
이 논문의 저자 정보
이 논문과 함께 이용한 논문
다양한 배양액 조성이 코이어 수경재배 딸기‘매향’의 생육과 수량에 미치는 영향
이정훈
,
이용범
,
최기영
시설원예·식물공장
2017
.07
새싹보리 열수 추출물의 혈행 개선 효과
이민희
,
김다경
,
박수정
외 5명
한국식품영양과학회지
2018
.01
Selection of model viruses for foot-and-mouth disease virus-related-experiments
Tae-Hwan Kim
,
Thilina U. B. He
,
ath
외 6명
미생물학회지
2017
.12
아마란스 안토시아닌의 안정성과 항산화 작용 조사
이진하
,
이혜빈
,
최선일
외 6명
한국식품영양과학회지
2017
.12
The involvement of ginseng berry extract in blood flow via regulation of blood coagulation in rats fed a high-fat diet
Min Hee Kim
,
Jongsung Lee
,
Sehyun Jung
외 3명
Journal of Ginseng Research
2017
.04
최근 본 자료
댓글(0)
0
UCI(KEPA) : I410-ECN-0101-2018-524-001599397