지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2018.1
- 수록면
- 75 - 80 (6page)
이용수
초록· 키워드
오류제보하기
Background: The aim of the present study was to evaluate the potential protective effects of six ginsenosides(Rb1, Rb2, Rc, Rd, Rg1, and Rg3) isolated from Panax ginseng against tacrolimus (FK506)-induced apoptosis in renal proximal tubular LLC-PK1 cells.
Methods: LLC-PK1 cells were treated with FK506 and ginsenosides, and cell viability was measured. Protein expressions of mitogen-activated protein kinases, caspase-3, and kidney injury molecule-1 (KIM-1) were evaluated by Western blotting analyses. The number of apoptotic cells was measured using an image-based cytometric assay.
Results: Reduction in cell viability by 60mM FK506 was ameliorated significantly by cotreatment with ginsenosides Rg1 and Rb1. The phosphorylation of p38, extracellular signal-regulated kinases, and KIM-1, and cleavage of caspase-3, increased markedly in LLC-PK1 cells treated with FK506 and significantly decreased after cotreatment with ginsenoside Rb1. The number of apoptotic cells decreased by 6.0% after cotreatment with ginsenoside Rb1 (10μM and 50μM).
Conclusion: The antiapoptotic effects of ginsenoside Rb1 on FK506-induced apoptosis were mediated by the inhibition of mitogen-activated protein kinases and caspase activation.
Methods: LLC-PK1 cells were treated with FK506 and ginsenosides, and cell viability was measured. Protein expressions of mitogen-activated protein kinases, caspase-3, and kidney injury molecule-1 (KIM-1) were evaluated by Western blotting analyses. The number of apoptotic cells was measured using an image-based cytometric assay.
Results: Reduction in cell viability by 60mM FK506 was ameliorated significantly by cotreatment with ginsenosides Rg1 and Rb1. The phosphorylation of p38, extracellular signal-regulated kinases, and KIM-1, and cleavage of caspase-3, increased markedly in LLC-PK1 cells treated with FK506 and significantly decreased after cotreatment with ginsenoside Rb1. The number of apoptotic cells decreased by 6.0% after cotreatment with ginsenoside Rb1 (10μM and 50μM).
Conclusion: The antiapoptotic effects of ginsenoside Rb1 on FK506-induced apoptosis were mediated by the inhibition of mitogen-activated protein kinases and caspase activation.
목차
ABSTRACT
1. Introduction
2. Materials and methods
3. Results and discussion
References
참고문헌 (42)
참고문헌 신청
[학술지(정기간행물)] - Wallemacq PE / 1993 / FK506 (tacrolimus), a novel immunosuppressant in organ transplantation: clinical, biomedical, and analytical aspects / Clin Chem 39 : 2219 ~ 2228
[학술지(정기간행물)] - Cosner D / 2015 / Proximal tubular injury in medullary rays is an early sign of acute tacrolimus nephrotoxicity / J Transplant 2015 : 142521 ~
[학술지(정기간행물)] - Dumont FJ / 2000 / FK506, an immunosuppressant targeting calcineurin function / Curr Med Chem 7 : 731 ~ 748
[학술지(정기간행물)] - Tada H / 2002 / Effects of thymic hormone on reactive oxygen speciesscavengers and renal function in tacrolimus-induced nephrotoxicity / Life Sci 70 : 1213 ~ 1223
[학술지(정기간행물)] - Al-Harbi NO / 2015 / Treatment with aliskiren ameliorates tacrolimus-induced nephrotoxicity in rats / J Renin Angiotensin Aldosterone Syst 16 : 1329 ~ 1336
[학술지(정기간행물)] - Cosner D / 2015 / Proximal tubular injury in medullary rays is an early sign of acute tacrolimus nephrotoxicity / J Transplant 2015 : 142521 ~
[학술지(정기간행물)] - Dumont FJ / 2000 / FK506, an immunosuppressant targeting calcineurin function / Curr Med Chem 7 : 731 ~ 748
[학술지(정기간행물)] - Tada H / 2002 / Effects of thymic hormone on reactive oxygen speciesscavengers and renal function in tacrolimus-induced nephrotoxicity / Life Sci 70 : 1213 ~ 1223
[학술지(정기간행물)] - Al-Harbi NO / 2015 / Treatment with aliskiren ameliorates tacrolimus-induced nephrotoxicity in rats / J Renin Angiotensin Aldosterone Syst 16 : 1329 ~ 1336
함께 읽어보면 좋을 논문
논문 유사도에 따라 DBpia 가 추천하는 논문입니다. 함께 보면 좋을 연관 논문을 확인해보세요!
-
Protective effect of Korean Red Ginseng against FK506-induced damage in LLC-PK1 cells
Journal of Ginseng Research
2017 .07
-
Biotransformation of major ginsenosides in ginsenoside model culture by lactic acid bacteria
Journal of Ginseng Research
2017 .01
-
Beneficial effects of fermented black ginseng and its ginsenoside 20(S)-Rg3 against cisplatin-induced nephrotoxicity in LLC-PK1 cells
Journal of Ginseng Research
2016 .04
-
Protective effect of ginsenoside Rh3 against anticancer drug-induced apoptosis in LLC-PK1 kidney cells
Journal of Ginseng Research
2017 .04
-
Kinetic study for the optimization of ginsenoside Rg3 production by heat treatment of ginsenoside Rb1
Journal of Ginseng Research
2015 .10
-
Ginsenoside Rb1 Inhibits Doxorubicin-Triggered H9C2 Cell Apoptosis via Aryl Hydrocarbon Receptor
Biomolecules & Therapeutics(구 응용약물학회지)
2017 .01
-
Proteomic analysis reveals that the protective effects of ginsenoside Rb1 are associated with the actin cytoskeleton in β-amyloid-treated neuronal cells
Journal of Ginseng Research
2016 .07
-
Molecular signaling of ginsenosides Rb1, Rg1, and Rg3 and their mode of actions
Journal of Ginseng Research
2018 .04
-
Ginsenoside Rb2 suppresses the glutamate-mediated oxidative stress and neuronal cell death in HT22 cells
Journal of Ginseng Research
2019 .04
-
Enzymatic formation of compound-K from ginsenoside Rb1 by enzyme preparation from cultured mycelia of Armillaria mellea
Journal of Ginseng Research
2016 .04
-
Ginsenoside Rb2: A review of pharmacokinetics and pharmacological effects
Journal of Ginseng Research
2022 .03
-
Ginsenoside Rb1함유 필름의 구강 내 창상 회복 촉진 효과
대한소아치과학회지
2022 .08
-
Variations in Ginsenosides of Raw Ginseng According to Heating Temperature and Time
Journal of Pharmacopuncture
2020 .01
-
Ginsenoside Rb1 and Rb2 upregulate Akt/mTOR signalingemediated muscular hypertrophy and myoblast differentiation
Journal of Ginseng Research
2020 .05
-
Effects of processing method on the pharmacokinetics and tissue distribution of orally administered ginseng
Journal of Ginseng Research
2018 .01
-
Cardioprotective effect of ginsenoside Rb1 via regulating metabolomics profiling and AMP-activated protein kinase-dependent mitophagy
Journal of Ginseng Research
2022 .03
-
Ginsenoside Rb1 increases macrophage phagocytosis through p38 mitogen-activated protein kinase/Akt pathway
Journal of Ginseng Research
2019 .06
-
Accumulation characteristics and correlation analysis of five ginsenosides with different cultivation ages from different regions
Journal of Ginseng Research
2015 .10
-
Ginsenoside Rb3 ameliorates podocyte injury under hyperlipidemic conditions via PPARδ- or SIRT6-mediated suppression of inflammation and oxidative stress
Journal of Ginseng Research
2023 .05
-
인삼의 열처리 과정 중 생성되는 3종의 수산화진세노사이드에 대한 연구
생약학회지(Korean Journal of Pharmacognosy)
2020 .12
이 논문의 저자 정보
최근 본 자료
댓글(0)
0
UCI(KEPA) : I410-ECN-0101-2018-524-001744572