Ginsenoside Rb1 Inhibits Doxorubicin-Triggered H9C2 Cell Apoptosis via Aryl Hydrocarbon Receptor

Zhang, Yaxin; Wang, Yuguang; Ma, Zengchun; Liang, Qiande; Tang, Xianglin; Tan, Hongling; Xiao, Chengrong; Gao, Yue

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자료유형: 학술저널

저자정보: Zhang, Yaxin (Department of Pharmacology and Toxicology, Beijing Institute of Radiation Medicine) Wang, Yuguang (Department of Pharmacology and Toxicology, Beijing Institute of Radiation Medicine) Ma, Zengchun (Department of Pharmacology and Toxicology, Beijing Institute of Radiation Medicine) Liang, Qiande (Department of Pharmacology and Toxicology, Beijing Institute of Radiation Medicine) Tang, Xianglin (Department of Pharmacology and Toxicology, Beijing Institute of Radiation Medicine) Tan, Hongling (Department of Pharmacology and Toxicology, Beijing Institute of Radiation Medicine) Xiao, Chengrong (Department of Pharmacology and Toxicology, Beijing Institute of Radiation Medicine) Gao, Yue (Department of Pharmacology and Toxicology, Beijing Institute of Radiation Medicine)

저널정보: 한국응용약물학회 Biomolecules & Therapeutics(구 응용약물학회지) Biomolecules & therapeutics 제25권 제2호

발행연도: 2017.1

수록면: 202 - 212 (11page)

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Doxorubicin (DOX) is a highly effective chemotherapeutic agent; however, the dose-dependent cardiotoxicity associated with DOX significantly limits its clinical application. In the present study, we investigated whether Rb1 could prevent DOX-induced apoptosis in H9C2 cells via aryl hydrocarbon receptor (AhR). H9C2 cells were treated with various concentrations ($-{\mu}M$) of Rb1. AhR, CYP1A protein and mRNA expression were quantified with Western blot and real-time PCR analyses. We also evaluated the expression levels of caspase-3 to assess the anti-apoptotic effects of Rb1. Our results showed that Rb1 attenuated DOX-induced cardiomyocytes injury and apoptosis and reduced caspase-3 and caspase-8, but not caspase-9 activity in DOX-treated H9C2 cells. Meanwhile, pre-treatment with Rb1 decreased the expression of caspase-3 and PARP in the protein levels, with no effects on cytochrome c, Bax, and Bcl-2 in DOX-stimulated cells. Rb1 markedly decreased the CYP1A1 and CYP1A2 expression induced by DOX. Furthermore, transfection with AhR siRNA or pre-treatment with AhR antagonist CH-223191 significantly inhibited the ability of Rb1 to decrease the induction of CYP1A, as well as caspase-3 protein levels following stimulation with DOX. In conclusion, these findings indicate that AhR plays an important role in the protection of Ginsenoside Rb1 against DOX-triggered apoptosis of H9C2 cells.

#Ginsenoside Rb1 #Doxorubicin #apoptosis #AhR #CYP1A

참고문헌 (41)

참고문헌 신청

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