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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2018.4
- 수록면
- 135 - 145 (11page)
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초록· 키워드
오류제보하기
Due to the critical location and physiological activities of the retinal pigment epithelial (RPE) cell, it is constantly subjected to contact with various infectious agents and inflammatory mediators. However, little is known about the signaling events in RPE involved in Toxoplasma gondii infection and development. The aim of the study is to screen the host mRNA transcriptional change of 3 inflammation-related gene categories, PI3K/Akt pathway regulatory components, blood vessel development factors and ROS regulators, to prove that PI3K/Akt or mTOR signaling pathway play an essential role in regulating the selected inflammation-related genes. The selected genes include PH domain and leucine- richrepeat protein phosphatases (PHLPP), casein kinase2 (CK2), vascular endothelial growth factor (VEGF), pigment epithelium-derived factor (PEDF), glutamate-cysteine ligase (GCL), glutathione S-transferase (GST), and NAD(P)H: quinone oxidoreductase (NQO1). Using reverse transcription polymerase chain reaction (RT-PCR) and quantitative real-time reverse transcription polymerase chain reaction (qRT-PCR), we found that T. gondii up-regulates PHLPP2, CK2β, VEGF, GCL, GST, and NQO1 gene expression levels, but down-regulates PHLPP1 and PEDF mRNA transcription levels. PI3K inhibition and mTOR inhibition by specific inhibitors showed that most of these host gene expression patterns were due to activation of PI3K/Akt or mTOR pathways with some exceptional cases. Taken together, our results reveal a new molecular mechanism of these gene expression change dependent on PI3K/Akt or mTOR pathways and highlight more systematical insight of how an intracellular T. gondii can manipulate host genes to avoid host defense.
목차
Abstract
INTRODUCTION
MATERIALS AND METHODS
RESULTS
DISCUSSION
REFERENCES
참고문헌 (41)
참고문헌 신청
[학술지(정기간행물)] - Vallochi AL / 2002 / Ocular toxoplasmosis: more than just what meets the eye / Scand J Immunol 55 : 324 ~ 328
[학술지(정기간행물)] - Rao NA / 1977 / Toxoplasmic retinochoroiditis: electron-microscopic and immunofluorescence studies of formalin-fixed tissue / Arch Ophthalmol 95 : 273 ~ 277
[학술지(정기간행물)] - Willermain F / 2002 / Retinal pigment epithelial cells phagocytosis of T lymphocytes: possible implication in the immune privilege of the eye / Br J Ophthalmol 86 : 1417 ~ 1421
[학술지(정기간행물)] - Sibley LD / 2003 / Toxoplasma gondii: perfecting an intracellular life style / Traffic 4 : 581 ~ 586
[학술지(정기간행물)] - Boothroyd JC / 2008 / Kiss and spit: the dual roles of Toxoplasma rhoptries / Nat Rev Microbiol 6 : 79 ~ 88
[학술지(정기간행물)] - Rao NA / 1977 / Toxoplasmic retinochoroiditis: electron-microscopic and immunofluorescence studies of formalin-fixed tissue / Arch Ophthalmol 95 : 273 ~ 277
[학술지(정기간행물)] - Willermain F / 2002 / Retinal pigment epithelial cells phagocytosis of T lymphocytes: possible implication in the immune privilege of the eye / Br J Ophthalmol 86 : 1417 ~ 1421
[학술지(정기간행물)] - Sibley LD / 2003 / Toxoplasma gondii: perfecting an intracellular life style / Traffic 4 : 581 ~ 586
[학술지(정기간행물)] - Boothroyd JC / 2008 / Kiss and spit: the dual roles of Toxoplasma rhoptries / Nat Rev Microbiol 6 : 79 ~ 88
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UCI(KEPA) : I410-ECN-0101-2018-513-002040232