지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2018.10
- 수록면
- 496 - 503 (8page)
이용수
초록· 키워드
오류제보하기
Background: Korean ginseng (Panax ginseng) plays an anti-inflammatory role in a variety of inflammatory diseases such as gastritis, hepatitis, and colitis. However, inflammation-regulatory activity of the calyx of the P. ginseng berry has not been thoroughly evaluated. To understand whether the calyx portion of the P. ginseng berry is able to ameliorate inflammatory processes, an ethanolic extract of P. ginseng berry calyx (Pg-C-EE)was prepared, and lipopolysaccharide-activated macrophages and HEK293 cells transfected with inflammation-regulatory proteins were used to test the anti-inflammatory action of Pg-C-EE.
Methods: The ginsenoside contents of Pg-C-EE were analyzed by HPLC. Suppressive activity of Pg-C-EE on NO production, inflammatory gene expression, transcriptional activation, and inflammation signaling events were examined using the Griess assay, reverse transcription-polymerization chain reaction, luciferase activity reporter gene assay, and immunoblotting analysis.
Results: Pg-C-EE reduced NO production and diminished mRNA expression of inflammatory genes such as cyclooxygenase-2, inducible NO synthase, and tumor necrosis factor-a in a dose-dependent manner. This extract suppressed luciferase activity induced only by nuclear factor-kB. Interestingly, immunoblotting analysis results demonstrated that Pg-C-EE reduced the activities of protein kinase B (AKT)1 and AKT2.
Conclusion: These results suggest that Pg-C-EE may have nuclear-factor-kB-targeted anti-inflammatory properties through suppression of AKT. The calyx of the P. ginseng berry is an underused part of the ginseng plant, and development of calyx-derived extracts may be useful for treatment of inflammatory diseases.
Methods: The ginsenoside contents of Pg-C-EE were analyzed by HPLC. Suppressive activity of Pg-C-EE on NO production, inflammatory gene expression, transcriptional activation, and inflammation signaling events were examined using the Griess assay, reverse transcription-polymerization chain reaction, luciferase activity reporter gene assay, and immunoblotting analysis.
Results: Pg-C-EE reduced NO production and diminished mRNA expression of inflammatory genes such as cyclooxygenase-2, inducible NO synthase, and tumor necrosis factor-a in a dose-dependent manner. This extract suppressed luciferase activity induced only by nuclear factor-kB. Interestingly, immunoblotting analysis results demonstrated that Pg-C-EE reduced the activities of protein kinase B (AKT)1 and AKT2.
Conclusion: These results suggest that Pg-C-EE may have nuclear-factor-kB-targeted anti-inflammatory properties through suppression of AKT. The calyx of the P. ginseng berry is an underused part of the ginseng plant, and development of calyx-derived extracts may be useful for treatment of inflammatory diseases.
목차
abstract
1. Introduction
2. Materials and methods
3. Results
4. Discussion
References
참고문헌 (52)
참고문헌 신청
[학술지(정기간행물)] - Zou J / 2015 / Lipopolysaccharide-induced tumor necrosis factor-${\alpha}$ factor enhances inflammation and is associated with cancer (Review) / Mol Med Rep 12 : 6399 ~ 6404
[학술지(정기간행물)] - Ferrero-Miliani L / 2007 / Chronic inflammation: importance of NOD2 and NALP3 in interleukin-$1{\beta}$ generation / Clin Exp Immunol 147 : 227 ~ 235
[학술지(정기간행물)] - Roberts-Thomson IC / 2011 / Cells, cytokines and inflammatory bowel disease: a clinical perspective / Expert Rev Gastroenterol Hepatol 5 : 703 ~ 716
[학술지(정기간행물)] - Mahla RS / 2013 / Sweeten PAMPs: role of sugar complexed PAMPs in innate immunity and vaccine biology / Front Immunol 4 : 248 ~
[학술지(정기간행물)] - Ribeiro-Gomes F / 2006 / Neutrophils, apoptosis and phagocytic clearance: an innate sequence of cellular responses regulating intramacrophagic parasite infections / Parasitology 132 : S61 ~ S68
[학술지(정기간행물)] - Ferrero-Miliani L / 2007 / Chronic inflammation: importance of NOD2 and NALP3 in interleukin-
[학술지(정기간행물)] - Roberts-Thomson IC / 2011 / Cells, cytokines and inflammatory bowel disease: a clinical perspective / Expert Rev Gastroenterol Hepatol 5 : 703 ~ 716
[학술지(정기간행물)] - Mahla RS / 2013 / Sweeten PAMPs: role of sugar complexed PAMPs in innate immunity and vaccine biology / Front Immunol 4 : 248 ~
[학술지(정기간행물)] - Ribeiro-Gomes F / 2006 / Neutrophils, apoptosis and phagocytic clearance: an innate sequence of cellular responses regulating intramacrophagic parasite infections / Parasitology 132 : S61 ~ S68
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