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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2018.4
- 수록면
- 113 - 118 (6page)
- DOI
- 10.7783/KJMCS.2018.26.2.113
이용수
초록· 키워드
오류제보하기
Background: Gastrodia elata Blume is a saprophytic perennial plant in the Orchidaceae family, because of its agricultural and medicinal effectiveness, researchers focus on its genome and chemical components. However, cytogenetic information based on the chromosome structure and composition to construct chromosomal backbone for genome sequencing research and for the development and breeding of plants is very limited.
Methods and Results: We determined the metaphase chromosome composition of the G. elata genome by fluorescence in situ hybridization (FISH) using 5S and 45S rDNAs and telomeric repeat probes. The nuclear genome of G. elata was organized into 2 n = 36, with relatively small (2.71 - 5.50㎛)chromosomes that showed gradual decrease in size. Conglutination phenomenon was observed among the metaphase chromosomes, and it was distinguished from that in other plant metaphase chromosome spreads. One pair of signal was detected for each 5S and 45S rDNA in the pericentromeric region and interstitial region on the short arm of chromosomes 10 and 4, respectively, and telomeric DNA signals were detected in the terminal region of most chromosomes.
Conclusions: To our knowledge, this is the first FISH chromosome composition result in G. elata and could be useful in more comprehensive molecular cytogenetic and genomic analyses as well as breeding programs of the medicinal plant G. elata.
Methods and Results: We determined the metaphase chromosome composition of the G. elata genome by fluorescence in situ hybridization (FISH) using 5S and 45S rDNAs and telomeric repeat probes. The nuclear genome of G. elata was organized into 2 n = 36, with relatively small (2.71 - 5.50㎛)chromosomes that showed gradual decrease in size. Conglutination phenomenon was observed among the metaphase chromosomes, and it was distinguished from that in other plant metaphase chromosome spreads. One pair of signal was detected for each 5S and 45S rDNA in the pericentromeric region and interstitial region on the short arm of chromosomes 10 and 4, respectively, and telomeric DNA signals were detected in the terminal region of most chromosomes.
Conclusions: To our knowledge, this is the first FISH chromosome composition result in G. elata and could be useful in more comprehensive molecular cytogenetic and genomic analyses as well as breeding programs of the medicinal plant G. elata.
목차
ABSTRACT
INTRODUCTION
MATERIALS AND METHODS
RESULTS AND DISCUSSION
REFERENCES
참고문헌 (34)
참고문헌 신청
Aoyama M / 2012 / Cytological studies on three species of Gastrodia, myco-heterotrophic orchids / Chromosome Botany 7:113~116
Aoyama M / 1986 / Karyomorphological studies in Gastrodia elata and G. confusa / La Kromosomo 42:1336~1340
Aoyama M / 2011 / Chromosome number of Gastrodia javanica(Blume) Lindl., a myco-heterotrophic orchid / Chromosome Science 14:39~40
Hadassah Roa Belandres / 2015 / FISH Karyotype and GISH Meiotic Pairing Analyses of a Stable Intergeneric Hybrid xBrassicoraphanus Line BB#5 / 원예과학기술지 33(1):83~92
Deni B. / 1995 / Encyclopedia of herbs and their uses / Dorling Kindersley :154~168
Aoyama M / 1986 / Karyomorphological studies in Gastrodia elata and G. confusa / La Kromosomo 42:1336~1340
Aoyama M / 2011 / Chromosome number of Gastrodia javanica(Blume) Lindl., a myco-heterotrophic orchid / Chromosome Science 14:39~40
Hadassah Roa Belandres / 2015 / FISH Karyotype and GISH Meiotic Pairing Analyses of a Stable Intergeneric Hybrid xBrassicoraphanus Line BB#5 / 원예과학기술지 33(1):83~92
Deni B. / 1995 / Encyclopedia of herbs and their uses / Dorling Kindersley :154~168
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