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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2008.1
- 수록면
- 95 - 100 (6page)
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Glyceraldehyde-3-phosphate dehydrogenase (GAPDH), a main enzyme in the glycolytic pathway, is involved in cellular energy production and regarded as a housekeeping gene. Previously, cytosolic GAPDH was selected as the most significantly abundant gene in EST library of sweetpotato suspension cells. In this study, a full-length of cDNA clone (IbGAPDH) encoding GAPDH was isolated from suspension-cultured cells of sweetpotato (Ipomoea babatas), and its expression was investigated with a view to understanding the physiological function of GAPDH in relation to environmental stresses. IbGAPDH encoded a 36.9 kDa polypeptide consisting of 337 amino acids. When the deduced amino acid of IbGAPDH was compared with other higher plants, IbGAPDH showed high homology with cytosolic GAPDH. The mRNA level of IbGAPDH significantly increased under environmental stresses, such as H2O2, MV and cold treatments. Among them, the transcript level of IbGAPDH gene was the highest under cold stress. Further investigation of the transcription level under 10℃ or 15℃ was performed with different tissues of sweetpotato. The transcription of IbGAPDH was increased by cold stress with tissue-specificity, moreover, showed different patterns according to temperature.
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참고문헌 (15)
참고문헌 신청
Baek D. / 2008 / Suppression of reactive oxygen species by glyceraldehyde- 3-phosphate dehydrogenase / Phytochemistry 69 : 333 ~ 338
Bullow L / 1999 / Induction of the maize GapC4 promoter in transgenic potato under anaerobiosis and in Erwinia carotovorainoculated tuber tissue / Molecular Plant-Microbe Int
Cerff R / 1982 / Structural diversity and differential light control of mRNAs coding for angiosperm glyceraldehyde-3-phosphate dehydrogenases / Proc Natl Acad Sci USA 79 : 76
Hancock J. T. / 2005 / Proteomic identification of glyceraldehyde-3-phosphate dehydrogenase as an inhibitory target of hydrogen peroxide in Arabidopsis / Plant 43 : 828 ~ 835
Jeong MJ / 2000 / Isolation and characterization of the gene encoding glyceraldehyde- 3-phosphate dehydrogenase / Biochemical and Biophysical Research Communication 278 : 192
Bullow L / 1999 / Induction of the maize GapC4 promoter in transgenic potato under anaerobiosis and in Erwinia carotovorainoculated tuber tissue / Molecular Plant-Microbe Int
Cerff R / 1982 / Structural diversity and differential light control of mRNAs coding for angiosperm glyceraldehyde-3-phosphate dehydrogenases / Proc Natl Acad Sci USA 79 : 76
Hancock J. T. / 2005 / Proteomic identification of glyceraldehyde-3-phosphate dehydrogenase as an inhibitory target of hydrogen peroxide in Arabidopsis / Plant 43 : 828 ~ 835
Jeong MJ / 2000 / Isolation and characterization of the gene encoding glyceraldehyde- 3-phosphate dehydrogenase / Biochemical and Biophysical Research Communication 278 : 192
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