지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
초록· 키워드
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Adipocytes affect obesity through the regulation of lipid metabolism. Physical loading is an important regulator of fat tissue. There are ongoing in vitro studies inducing mechanotransduction on 3T3-L1 preadipocytes with mechanical stimulus in order to treat obesity by inhibiting adipogenesis and provoking cell death. In this study, our goal was to suggest a new therapy for obesity by investigating whether fluid shear stress (FSS) changes transcription factors on 3T3-L1 related with adipogenesis and cell death. FSS loading was applied to 3T3-L1 preadipocytes at 1Pa and 1Hz. After loading, bright field images were taken and an immunofluorescence assay was conducted to observe actin stress fiber formation. Western blot analysis was conducted to identify the activation of the ERK pathway as well as the adipogenic factors, which including C/EBPs and PPARγ. The expression of osteopontin, a protein related to inflammation in adipose tissue, and cell death related factors, Bax, Bcl-2, and Beclin, were also measured. Results showed that FSS stimulated the formation of actin stress fibers in 3T3-L1 and also that the activation of C/EBPs decreased significantly when compared with the control group. PPARγ activation in the 2 hour FSS group was lower than the 1 hour FSS group, which implied that the results were time dependent. Additionally, there were no differences in the expression of cell death factors after FSS loading. In summary, similar to other fibroblasts, the formation of actin stress fibers induced by mechanotransduction may affect the differentiation of 3T3-L1, leading to inhibition of adipogenesis and inflammation.
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참고문헌 (31)
참고문헌 신청
B.M. Spiegelman / 2001 / Obesity and the regulation of energy balance / cell 104(4):531~543
X. Formiguera / 2004 / Obesity: epidemiology and clinical aspects / Best Pract. Res. Clin. Gastroenterol. 18(6):1125~1146
C. Weyer / 2000 / Enlarged subcutaneous abdominal adipocyte size, but not obesity itself, predicts type II diabetes independent of insulin resistance / Diabetologia 43(12):1498~1506
Y.W. Wang / 2004 / Conjugated linoleic acid and obesity control: efficacy and mechanisms / Int. J. Obes. 28(8):941~955
C.L. Hsu / 2007 / Effects of capsaicin on induction of apoptosis and inhibition of adipogenesis in 3T3-L1 cells / J. Agric. Food Chem. 55(5):1730~1736
X. Formiguera / 2004 / Obesity: epidemiology and clinical aspects / Best Pract. Res. Clin. Gastroenterol. 18(6):1125~1146
C. Weyer / 2000 / Enlarged subcutaneous abdominal adipocyte size, but not obesity itself, predicts type II diabetes independent of insulin resistance / Diabetologia 43(12):1498~1506
Y.W. Wang / 2004 / Conjugated linoleic acid and obesity control: efficacy and mechanisms / Int. J. Obes. 28(8):941~955
C.L. Hsu / 2007 / Effects of capsaicin on induction of apoptosis and inhibition of adipogenesis in 3T3-L1 cells / J. Agric. Food Chem. 55(5):1730~1736
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