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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2010.1
- 수록면
- 697 - 701 (5page)
이용수
초록· 키워드
오류제보하기
Background : The red blood cell (RBC) deformability test is a useful method for measuring the ability of RBCs to adapt their shape to the flow conditions. Using this test, several investigators have shown the relationship between RBC deformability and numerous clinical conditions. For the quality control (QC) of RBC deformability test, we evaluated whether frozen-thawed-deglycerolized RBCs can be used as QC materials.
Methods : Packed RBCs were frozen with 40% (wt/vol) glycerol and stored at -80℃ for 3 months. For 10 different frozen RBC panels, RBCs were thawed, deglycerolized and stored at 4℃ for 4 weeks. Using microfluidic ektacytometer, we measured RBC deformability of the thawed RBCs. The stability of thawed RBCs was tested once a day for 28 days of storage time and was analyzed by simple regression analysis. The precision of the test using thawed RBCs was analyzed for 7 days of storage time by calculation of CV values of intra-assay (10 measurements/assay) and betweenday measurements.
Results : Frozen-thawed-deglycerolized RBCs were stable for 1 week. Within-run and betweenday precisions of the RBC deformability test during 7 days of storage of thawed RBCs were 1.4- 2.9%, and 1.9-2.8%, respectively.
Conclusions : Frozen-thawed-deglycerolized RBCs used in RBC deformability test showed satisfactory within-run and between-run precisions and stability for one week after thawing, and may be used as QC materials for this test. (Korean J Lab Med 2010;30:697-701)
목차
등록된 정보가 없습니다.
참고문헌 (14)
참고문헌 신청
Kim YK / 2008 / Susceptibility of oxidative stress on red blood cells exposed to gamma rays: hemorheological evaluation / Clin Hemorheol Microcirc 40 : 315 ~ 324
Morgan LO / 1978 / A whole blood control for the Coulter Model S / J Clin Pathol 31 : 50 ~ 53
Plebani M / 2002 / Erythrocyte sedimentation rate: use of fresh blood for quality control / Am J Clin Pathol 117 : 621 ~ 626
Scott KL / 2005 / Biopreservation of red blood cells: past, present, and future / Transfus Med Rev 19 : 127 ~ 142
Shin S / 2005 / Rapid cell-deformability sensing system based on slit-flow laser diffractometry with decreasing pressure differential / Biosens Bioelectron 20 : 1291 ~ 1297
Morgan LO / 1978 / A whole blood control for the Coulter Model S / J Clin Pathol 31 : 50 ~ 53
Plebani M / 2002 / Erythrocyte sedimentation rate: use of fresh blood for quality control / Am J Clin Pathol 117 : 621 ~ 626
Scott KL / 2005 / Biopreservation of red blood cells: past, present, and future / Transfus Med Rev 19 : 127 ~ 142
Shin S / 2005 / Rapid cell-deformability sensing system based on slit-flow laser diffractometry with decreasing pressure differential / Biosens Bioelectron 20 : 1291 ~ 1297
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