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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
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Objectives: Gilgyung-tang (GGT) has been used as one of the main multi-herb formulas to treat “Peo-ong” (lung abscess). In this study, we investigated the inhibitory effects of water extracts of GGT on cell migration and invasion, two critical cellular processes that are often deregulated during metastasis, in human bladder cancer 5637 cells.
Methods: Effects on cell viability were quantified using an MTT assay. To analyze the anti-metastatic effects, we conducted a wound healing migration assay, an in vitro invasiveness assay, and a measurement of the transepithelial electrical resistance (TER). The expression of protein and mRNA were measured by Western blotting and real-time polymerase chain reaction (RT-PCR), respectively.
Results: GGT markedly inhibited the cell motility and invasiveness of 5637 cells within the concentration range that was not cytotoxic. The inhibitory effects of GGT on cell invasiveness were associated with tightening of the tight junctions (TJs), which was demonstrated by an increase in the TER. The RT-PCR and Western blotting results indicated that GGT decreased the levels of claudin proteins. GGT also inhibited the activity and expression of matrix metalloproteinase (MMP)-2 and -9 and simultaneously increased the levels of tissue inhibitor of metalloproteinase-1 and -2.
Conclusions: Our findings suggest that GGT reduces both the migration and the invasion of 5637 cells by modulating the activity of TJs and MMPs.
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참고문헌 (31)
참고문헌 신청
Vihinen P / 2005 / Matrix metalloproteinases as therapeutic targets in cancer / Curr Cancer Drug Targets 5(3):203~220
John A / 2001 / The role of matrix metalloproteinases in tumor angiogenesis and tumor metastasis / Pathol Oncol Res 7(1):14~23
Gibbs DF / 1999 / Characterization of matrix metalloproteinases produced by rat alveolar macrophages / Am J Respir Cell Mol Biol 20(6):1136~1144
Mook OR / 2004 / The role of gelatinases in colorectal cancer progression and metastasis / Biochim Biophys Acta 1705(2):69~89
Lombard C / 2005 / Assays of matrix metalloproteinases (MMPs) activities: a review / Biochimie 87(3-4):265~272
John A / 2001 / The role of matrix metalloproteinases in tumor angiogenesis and tumor metastasis / Pathol Oncol Res 7(1):14~23
Gibbs DF / 1999 / Characterization of matrix metalloproteinases produced by rat alveolar macrophages / Am J Respir Cell Mol Biol 20(6):1136~1144
Mook OR / 2004 / The role of gelatinases in colorectal cancer progression and metastasis / Biochim Biophys Acta 1705(2):69~89
Lombard C / 2005 / Assays of matrix metalloproteinases (MMPs) activities: a review / Biochimie 87(3-4):265~272
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