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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2010.1
- 수록면
- 91 - 97 (7page)
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Background: The aim of this study was to assess the feasibility of targeted ultrasound imaging on apoptosis with annexin A5microbubbles (A5MB) in acute doxorubicin-induced cardiotoxicity.
Methods: Avidinated and octafluoropropan-filled phospholipid microbubbles were conjugated with biotinylated annexin A5.
To confirm the specific binding of A5MB, flow cytometry was performed with hydrogen peroxide induced apoptosis in rat aorta smooth muscle cells incubated with fluorescein-5-isothiocyanate (FITC) labeled annexin A5 and A5MB. Adult male rats were injected intraperitoneally with 5 mg/kg doxorubicin weekly for 3 weeks (n = 5). Control rats were injected with normal saline (n = 5). At 24 hours after the final treatment, triggering imaging was performed 15 min after an intravenous bolus injection of A5MB for washout of freely circulating microbubbles. After echocardiography, the heart was isolated for histological detection of apoptosis by terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling (TUNEL) assay.
Results: In the in vitro tests, fluorescence intensity was low for healthy cells and high for apoptotic cells when incubated with FITC-labeled annexin A5 and A5MB. Rats treated with doxorubicin showed significant contrast opacification of the myocardium on contrast echocardiography using A5MB. However, no opacification was observed in control rats. Apoptosis was confirmed by TUNEL assay in doxorubicin treated rats.
Conclusion: Acute doxorubicin-induced cardiomyopathy based on early apoptosis can be assessed and imaged with targeted ultrasound imaging using A5MB in rats.
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참고문헌 (21)
참고문헌 신청
Arola OJ / 2000 / Acute doxorubicin cardiotoxicity involves cardiomyocyte apoptosis / Cancer Res 60 : 1789 ~ 1792
Bayne K / 1996 / Revised Guide for the Care and Use of Laboratory Animals available. American Physiological Society / Physiologist 39 : 199 ~
Bennink RJ / 2004 / Annexin V imaging of acute doxorubicin cardiotoxicity (apoptosis) in rats / J Nucl Med 45 : 842 ~ 848
Bristow MR / 1978 / Clinical spectrum of anthracycline antibiotic cardiotoxicity / Cancer Treat Rep 62 : 873 ~ 879
Dumont EA / 2001 / Real-time imaging of apoptotic cellmembrane changes at the single-cell level in the beating murine heart / Nat Med 7 : 1352 ~ 1355
Bayne K / 1996 / Revised Guide for the Care and Use of Laboratory Animals available. American Physiological Society / Physiologist 39 : 199 ~
Bennink RJ / 2004 / Annexin V imaging of acute doxorubicin cardiotoxicity (apoptosis) in rats / J Nucl Med 45 : 842 ~ 848
Bristow MR / 1978 / Clinical spectrum of anthracycline antibiotic cardiotoxicity / Cancer Treat Rep 62 : 873 ~ 879
Dumont EA / 2001 / Real-time imaging of apoptotic cellmembrane changes at the single-cell level in the beating murine heart / Nat Med 7 : 1352 ~ 1355
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