Antioxidant and Anti-Apoptotic Effect of Melatonin on the Vestibular Hair Cells of Rat Utricles

김정범; 정재윤; 안진철; 이정구; 황희준

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자료유형: 학술저널

저자정보: 김정범 정재윤 안진철 이정구 황희준

저널정보: 대한이비인후과학회 Clinical and Experimental Otorhinolaryngology Clinical and Experimental Otorhinolaryngology 제2권 제1호

발행연도: 2009.1

수록면: 6 - 12 (7page)

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Objectives. Aminoglycosides are commonly used antibiotic agents, and they are known to generate free oxygen radicals within the inner ear and to cause vestibulo-cochlear toxicity and permanent damage to the sensory hair cells and neurons. Melatonin, a pineal secretory product, has the properties of being a powerful direct and indirect antioxidant. The aim of the present study was to prove the antioxidant effect of melatonin against gentamicin-induced ototoxicty. Methods. The utricular maculae of Sprague-Dawley rats were prepared from postnatal day 2-4, and these maculae were were divided into 6 groups as follows: 1) control, 2) melatonin only, 3) gentamicin only, and 4), 5), and 6) gentamicin plus melatonin (10, 50, and 100 μM, respectively). To count the number of hair cells, 5 utricles from each group were stained with phalloidin-FITC on the 1st, 4th, and 7th days after drug administration. Reactive oxygen species (ROS) was assessed by using the fluorescent probe hydrofluorescent diacetate acetyl ester. The caspase-3 activity was also examined with using the fluorescent caspase-3 substrate and performing Western blotting. Results. The result of this study showed that gentamicin induced the loss of utricular hair cells, and this loss of hair cells was significantly attenuated by co-administration of melatonin. Melatonin reduced ROS production and caspase-3 activation in the gentamicin treated utricular hair cells. Conclusion. Our findings conclusively reveal that melatonin has protective effects against gentamicin-induced hair cell loss in the utricles of rat by inhibiting both ROS production and caspase-3 activity.

#Melatonin #Ototoxicity #Antioxidants #Utricle

참고문헌 (19)

참고문헌 신청

Cohen GM / 1997 / Caspases: the executioners of apoptosis / Biochem J. 326 : 1 ~ 16

Costa EJ / 1995 / Permeability of pure lipid bilayers to melatonin / J Pineal Res. 19 (3) : 123 ~ 126

Cunningham LL / 2004 / Overexpression of Bcl-2 prevents neomycin-induced hair cell death and caspase-9 activation in the adult mouse utricle in vitro / J Neurobiol 60 (1) :

Darlington CL / 2003 / Vestibulotoxicity following aminoglycoside antibiotics and its prevention / Curr Opin Investig Drugs 4 (7) : 841 ~ 846

Forge A / 2000 / Aminoglycoside antibiotics / Audiol Neurootol 5 (1) : 3 ~ 22

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